Optical biosensors for environmental monitoring based on computational and biotechnological tools for engineering the photosynthetic D1 protein of C. reinhardtii. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Optical biosensors for environmental monitoring based on computational and biotechnological tools for engineering the photosynthetic D1 protein of C. reinhardtii. (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.bios.2009.07.003 (literal)

Alternative label

• MT Giardi (a), V. Scognamiglio (a), G. Rea (a), G. Rodio (a), A. Antonacci (a), M. Lambreva (a), G. Pezzotti (b), U. Johanningmeier (c) (2009)
  Optical biosensors for environmental monitoring based on computational and biotechnological tools for engineering the photosynthetic D1 protein of C. reinhardtii.
  in Biosensors & bioelectronics
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• MT Giardi (a), V. Scognamiglio (a), G. Rea (a), G. Rodio (a), A. Antonacci (a), M. Lambreva (a), G. Pezzotti (b), U. Johanningmeier (c) (literal)

Pagina inizio

• 294 (literal)

Pagina fine

• 300 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.elsevier.com/locate/bios (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 25 (literal)

Rivista

• Biosensors & bioelectronics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 7 (literal)

Note

• Scopu (literal)
• ISI Web of Science (WOS) (literal)
• Google Scholar (literal)
• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• a) Institute of Crystallography, Area of Research of Rome, Department of Agrofood, CNR, Via Salaria km 29.300, 00015, Monterotondo Scalo, Rome, Italy; b) Biosensor srl, Via Palombara Sabina, km 18.4, Rome, Italy; c) Institute for Plant Physiology, Martin-Luther University, Weinbergweg 10, 06120 Halle, Germany; (literal)

Titolo

• Optical biosensors for environmental monitoring based on computational and biotechnological tools for engineering the photosynthetic D1 protein of C. reinhardtii. (literal)

Abstract

• Homology-based protein modelling and computational screening followed by virtual mutagenesis analyseswere used to identify functional amino acids in the D1 protein of the photosynthetic electron transfer chain interacting with herbicides. A library of functional mutations in the unicellular green alga Chlamydomonas reinhardtii for preparing biomediators was built and their interactions with herbicides were calculated. D1 proteins giving the lowest and highest binding energy with herbicides were considered as suitable for preparing the environmental biosensors for detecting specific herbicide classes. Arising from the results of theoretical calculations, three mutants were prepared by site-directed mutagenesis and characterized by fluorescence analysis. Their adsorption and selective recognition ability were studied by an equilibrium-adsorption method. The S268C and S264K biomediators showed high sensitivity and resistance, respectively, to both triazine and urea classes of herbicides. When immobilized on a silicon septum, the biomediators were found to be highly stable, remaining so for at least 1-month at room temperature. The fluorescence properties were exploited and a reusable and portable multiarray optical biosensor for environmental monitoring was developed with limits of detection between 0.8×10-11 and 3.0×10-9, depending on the target analyte. In addition, biomediator regeneration without obvious deterioration in performance was demonstrated (literal)

Prodotto di

• Institute of Crystallography (IC) (Istituto)
• Sintesi sostenibile di composti bioattivi e per biosensing tramite biofarms (PM.P04.013.001) (Modulo)

Autore CNR

• VIVIANA SCOGNAMIGLIO (Unità di personale interno)
• AMINA ANTONACCI (Persona)
• GIUSEPPINA REA (Unità di personale interno)
• Giuseppe RODIO (Persona)
• MAYA LAMBREVA (Unità di personale esterno)
• MARIA TERESA GIARDI (Unità di personale interno)

Incoming links:

Autore CNR di

• MARIA TERESA GIARDI (Unità di personale interno)
• GIUSEPPINA REA (Unità di personale interno)
• Giuseppe RODIO (Persona)
• AMINA ANTONACCI (Persona)
• MAYA LAMBREVA (Unità di personale esterno)
• VIVIANA SCOGNAMIGLIO (Unità di personale interno)

Prodotto

• Institute of Crystallography (IC) (Istituto)
• Sintesi sostenibile di composti bioattivi e per biosensing tramite biofarms (PM.P04.013.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biosensors & bioelectronics (Rivista)