The Mycovirus CHV1 Disrupts Secretion of a Developmentally Regulated Protein in Cryphonectria parasitica (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• The Mycovirus CHV1 Disrupts Secretion of a Developmentally Regulated Protein in Cryphonectria parasitica (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1128/JVI.05756-11 (literal)

Alternative label

• Kazmierczak P., McCabe P., Turina M., Jacob-Wilk D., Van Alfen N.K. (2012)
  The Mycovirus CHV1 Disrupts Secretion of a Developmentally Regulated Protein in Cryphonectria parasitica
  in Journal of virology (Print); ASM, American society for microbiology, Washington, DC (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Kazmierczak P., McCabe P., Turina M., Jacob-Wilk D., Van Alfen N.K. (literal)

Pagina inizio

• 6067 (literal)

Pagina fine

• 6074 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3372201/pdf/zjv6067.pdf (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 86 (literal)

Rivista

• Journal of virology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 11 (literal)

Note

• ubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• K.P., McB. P., J.W.D. & V.A.N.K.: DEPARTMENT OF PLANT PATHOLOGY, COLLEGE OF AGRICULTURAL AND ENVIRONMENTAL SCIENCES, UNIVERSITY OF CALIFORNIA, DAVIS, CA, USA T.M.: ISTITUTO DI VIROLOGIA GìVEGETALE, CNR, TORINO, ITALY (literal)

Titolo

• The Mycovirus CHV1 Disrupts Secretion of a Developmentally Regulated Protein in Cryphonectria parasitica (literal)

Abstract

• Infection of the chestnut blight fungus Cryphonectria parasitica with Cryphonectria hypovirus 1 (CHV1) causes disruption of virulence, pigmentation, and sporulation. Transcriptional downregulation of key developmentally regulated fungal genes occurs during infection, but vegetative growth is unaffected. Previous studies showed that CHV1 utilizes trans-Golgi network (TGN) secretory vesicles for replication. In this study, the fungal cell surface hydrophobin cryparin was chosen as a marker to follow secretion in virally infected and noninfected strains. Subcellular fractionation, cryparin-green fluorescent protein (GFP) fusion, and Western blot studies confirmed that vesicles containing cryparin copurify with the same fractions previously shown to contain elements of the viral replication complex and the TGN resident endoprotease Kex2. This vesicle fraction accumulated to a much greater concentration in the CHV1-infected strains than in noninfected strains. Pulse-chase analysis showed that the rates and amount of cryparin being secreted by the CHV1 containing strains was much lower than in noninfected strains, and the dwell time of cryparin within the cell after labeling was significantly greater in the CHV1-infected strains than in the noninfected ones. These results suggest that the virus perturbs a specific late TGN secretory pathway resulting in buildup of a key protein important for fungal development. (literal)

Editore

• ASM, American society for microbiology (Editore)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• MASSIMO TURINA (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "The Mycovirus CHV1 Disrupts Secretion of a Developmentally Regulated Protein in Cryphonectria parasitica" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MASSIMO TURINA (Unità di personale interno)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of virology (Rivista)

Editore di

• ASM, American society for microbiology (Editore)

Insieme di parole chiave di

• Parole chiave di "The Mycovirus CHV1 Disrupts Secretion of a Developmentally Regulated Protein in Cryphonectria parasitica" (Insieme di parole chiave)