Search for Cellular Stress Biomarkers in Lymphocytes from Patients with Multiple Sclerosis: A Pilot Study (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Search for Cellular Stress Biomarkers in Lymphocytes from Patients with Multiple Sclerosis: A Pilot Study (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1371/journal.pone.0044935 (literal)

Alternative label

• Grecchi S, Mazzini G, Lisa A, Armentero MT, Bergamaschi R, Romani A, Blandini F, Di Perri C, Scovassi AI (2012)
  Search for Cellular Stress Biomarkers in Lymphocytes from Patients with Multiple Sclerosis: A Pilot Study
  in PloS one
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Grecchi S, Mazzini G, Lisa A, Armentero MT, Bergamaschi R, Romani A, Blandini F, Di Perri C, Scovassi AI (literal)

Pagina inizio

• e44935 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 7 (literal)

Rivista

• PloS one (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• IGM CNR; IRCCS FONDAZIONE MONDINO, Pavia (literal)

Titolo

• Search for Cellular Stress Biomarkers in Lymphocytes from Patients with Multiple Sclerosis: A Pilot Study (literal)

Abstract

• Multiple Sclerosis (MS) is a chronic disease of the central nervous system, the etiology of which, although not completely known, involves inflammation and autoimmunity. In the present study we aimed at identifying molecular markers of apoptosis, cellular stress and DNA damage in isolated peripheral blood mononuclear cells (PBMCs) of MS patients. The analysis was carried on 19 relapsing-remitting untreated MS patients and 13 healthy individuals. We investigated the emergency-driven synthesis of poly(ADP-ribose) (PAR), the expression level of the constitutive enzyme poly(ADP-ribose) polymerase-1 (PARP-1) and the DNA damage-induced phosphorylation of histone H2AX. PAR accumulation, PARP-1 and phosphorylated H2AX (cH2AX) were detected by immunofluorescence experiments on PBMCs isolated from 19 patients and 13 healthy volunteers. Our results show for the first time a net increased amount in PAR and cH2AX in MS patients compared to healthy individuals. Patients were further subdivided in three groups, according to the neuroimaging (MRI)- based classification of disease phase. Remarkably, we found a positive correlation between the level of cH2AX and MS aggressiveness. In addition, apoptosis in PBMCs was monitored by flow cytometry of both phosphatidylserine exposure (revealed by Annexin V-FITC labeling) and membrane permeability to propidium iodide. Our observations provide the evidence that the number of apoptotic cells was significantly higher in patients compared to healthy individuals, thus suggesting that apoptosis could affect MS lymphocyte function. (literal)

Prodotto di

• Identificazione di composti con potenziale attività antitumorale (ME.P04.004.003) (Modulo)
• Sviluppo di nuove tecniche e metodologie ottiche per biologia di base e applicazioni biomediche (ME.P04.004.002) (Modulo)
• Biologia Computazionale (ME.P07.005.001) (Modulo)
• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• GIULIANO MAZZINI (Persona)
• ANTONELLA LISA (Persona)
• ANNA SCOVASSI (Unità di personale interno)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Biologia Computazionale (ME.P07.005.001) (Modulo)
• Identificazione di composti con potenziale attività antitumorale (ME.P04.004.003) (Modulo)
• Sviluppo di nuove tecniche e metodologie ottiche per biologia di base e applicazioni biomediche (ME.P04.004.002) (Modulo)

Autore CNR di

• ANNA SCOVASSI (Unità di personale interno)
• ANTONELLA LISA (Persona)
• GIULIANO MAZZINI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• PloS one (Rivista)