Activity and regulation of archaeal DNA alkyltransferase: conserved protein involved in repair of DNA alkylation damage. (Articolo in rivista)

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Label
  • Activity and regulation of archaeal DNA alkyltransferase: conserved protein involved in repair of DNA alkylation damage. (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1074/jbc.M111.308320 (literal)
Alternative label
  • Giuseppe Perugino, Antonella Vettone, Giuseppina Illiano, Anna Valenti, Maria C. Ferrara, Mosè Rossi and Maria Ciaramella1 (2012)
    Activity and regulation of archaeal DNA alkyltransferase: conserved protein involved in repair of DNA alkylation damage.
    in The Journal of biological chemistry (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Giuseppe Perugino, Antonella Vettone, Giuseppina Illiano, Anna Valenti, Maria C. Ferrara, Mosè Rossi and Maria Ciaramella1 (literal)
Pagina inizio
  • 4222 (literal)
Pagina fine
  • 31 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.jbc.org/content/287/6/4222.long (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 287 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 10 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 6 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Protein Biochemistry, Consiglio Nazionale delle Ricerche, Via P. Castellino 111, 80131 Naples, Italy. (literal)
Titolo
  • Activity and regulation of archaeal DNA alkyltransferase: conserved protein involved in repair of DNA alkylation damage. (literal)
Abstract
  • Agents that form methylation adducts in DNA are highly mutagenic and carcinogenic, and organisms have evolved specialized cellular pathways devoted to their repair, including DNA alkyltransferases. These are proteins conserved in eucarya, bacteria and archaea, acting by a unique reaction mechanism, which leads to direct repair of DNA alkylation damage and irreversible protein alkylation. The alkylated form of DNA alkyltransferases is inactive, and in eukaryotes, it is rapidly directed to degradation. We report here in vitro and in vivo studies on the DNA alkyltransferase from the thermophilic archaeon Sulfolobus solfataricus (SsOGT). The development of a novel, simple, and sensitive fluorescence-based assay allowed a careful characterization of the SsOGT biochemical and DNA binding activities. In addition, transcriptional and post-translational regulation of SsOGT by DNA damage was studied. We show that although the gene transcription is induced by alkylating agent treatment, the protein is degraded in vivo by an alkylation-dependent mechanism. These experiments suggest a striking conservation, from archaea to humans, of this important pathway safeguarding genome stability. (literal)
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