The accumulation of MMS-induced single strand breaks in G(1) phase is recombinogenic in DNA polymerase beta defective mammalian cells (Articolo in rivista)

Type
Label
  • The accumulation of MMS-induced single strand breaks in G(1) phase is recombinogenic in DNA polymerase beta defective mammalian cells (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1093/nar/gki168 (literal)
Alternative label
  • Pascucci B. ; Russo M.T. ; Crescenzi M. ; Bignami M. ; Eugenia Dogliotti (2005)
    The accumulation of MMS-induced single strand breaks in G(1) phase is recombinogenic in DNA polymerase beta defective mammalian cells
    in Nucleic acids research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Pascucci B. ; Russo M.T. ; Crescenzi M. ; Bignami M. ; Eugenia Dogliotti (literal)
Pagina inizio
  • 280 (literal)
Pagina fine
  • 288 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 33 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Pascucci B.: Istituto di Cristallografia, CNR, Sezione di Roma, PO Box 10, 00016 Monterotondo Stazione, Roma, Italy Russo M.T., Crescenzi M., Bignami M. and Dogliotti E.: Department of Environment and Primary Prevention, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy (literal)
Titolo
  • The accumulation of MMS-induced single strand breaks in G(1) phase is recombinogenic in DNA polymerase beta defective mammalian cells (literal)
Abstract
  • DNA polymerase (Pol) b null mouse embryonic fibroblasts provide a useful cell system to investigate the effects of alterations in base excision repair (BER) on genome stability. These cells are characterized by hypersensitivity to the cytotoxic effects of methyl methanesulfonate (MMS) and by decreased repair of the MMS-induced DNA single strand breaks (SSB). Here,weshowthat, in theabsence of Polb,SSB accumulate in G1 phase cells, accompanied by the formation of proliferating cell nuclear antigen foci in the nuclei.Whenreplicating Pol b null cells are treated with MMS, a rapid phosphorylation of histone H2AX is detected in the nuclei of S phase cells, indicating that double strand breaks (DSB) are formed in response to unrepaired SSB. This is followed by relocalization within the nuclei of Rad51 protein, which is essential for homologous recombination (HR). These findings are compatible with a model where, in mammalian cells, unrepaired SSB produced during BER are substrates for the HR pathway via DSB formation. This is an example of a coordinated effort of two different repair pathways, BER and HR, to protect mammalian cells from alkylation-induced cytotoxicity. (literal)
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