http://www.cnr.it/ontology/cnr/individuo/prodotto/ID197773

Detection of odorant molecules via surface acoustic wave biosensor array based on odorant-binding proteins (Articolo in rivista)

Type

Label

Detection of odorant molecules via surface acoustic wave biosensor array based on odorant-binding proteins (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

F. Di Pietrantonio a, D. Cannatà a, M. Benetti a, E. Verona a, A. Varriale b, M. Staiano b, S. D'Auria b (2012)
Detection of odorant molecules via surface acoustic wave biosensor array based on odorant-binding proteins
in Biosensors & bioelectronics
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

F. Di Pietrantonio a, D. Cannatà a, M. Benetti a, E. Verona a, A. Varriale b, M. Staiano b, S. D'Auria b (literal)

Pagina inizio

Pagina fine

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

Rivista

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

a Institute of Acoustics and Sensors \"O.M.Corbino\", National Research Council of Italy, Via del Fosso del Cavaliere 100, 00133 Rome, Italy b Institute of Protein Biochemistry, National Research Council of Italy, Via Pietro Castellino 111, 80131 Naples, Italy (literal)

Titolo

Detection of odorant molecules via surface acoustic wave biosensor array based on odorant-binding proteins (literal)

Abstract

In this paper, we present an array of biosensors for vapour phase detection of odorant molecules based on surface acoustic wave (SAW) resonators coated with odorant-binding proteins (OBPs). For the first time, the sensing capabilities of three different OBPs, as sensitive layers for SAW devices, are studied and compared. The SAW biosensor array is composed of three SAW devices coated by the droplet method with the wild-type OBP from cow (wtbOBP), a double mutant of the OBP from cow (dmbOBP) and the wild-type OBP from pig (wtpOBP). An uncoated device is used to compensate the variations of the environmental parameters. The SAW devices consist of two-port resonators fabricated on quartz (ST-cut, x propagation) with electrodes made of aluminium covered with a thin gold film (2 nm thick). The obtained surface densities of OBP layers are between 1.18×10-6 kg/m2 and 2.31×10-6 kg/m2 and were calculated measuring the resonant frequency shift of the SAW devices after the coating. The SAW biosensor array was tested in nitrogen upon exposure to vapours of R-(-)-1-octen-3-ol (octenol), in the range of concentration between 13 and 61 ppm, and R-(-)-carvone (carvone), in the range between 9 and 80 ppm. The highest sensitivity for detection of octenol (25.9 Hz/ppm) was obtained using the wtpOBP-based SAW biosensor, while the highest sensitivity for detection of carvone (9.2 Hz/ppm) was obtained using the dmbOBP-based SAW biosensor. (literal)

Prodotto di

Autore CNR

Insieme di parole chiave

Autore CNR di

Prodotto

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

Insieme di parole chiave di

data.CNR.it