http://www.cnr.it/ontology/cnr/individuo/prodotto/ID196993

Anandamide, an endogenous cannabinomimetic substance, modulates rat brain protein kinase C in vitro (Articolo in rivista)

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Anandamide, an endogenous cannabinomimetic substance, modulates rat brain protein kinase C in vitro (Articolo in rivista) (literal)

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De Petrocellis L, Orlando P, Di Marzo V. (1995)
Anandamide, an endogenous cannabinomimetic substance, modulates rat brain protein kinase C in vitro
in Biochemistry and molecular biology international; Taylor & Francis Inc., Philadelphia (Stati Uniti d'America)
(literal)

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1. CNR, IST CHIM MOLEC INTERESSE BIOL, I-80072 ARCO FELICE, ITALY 2. CNR, IST CIBERNETICA, I-80072 ARCO FELICE, ITALY 3. CNR, IST BIOCHIM PROT & ENZIMOL, I-80072 ARCO, ITALY (literal)

Titolo

Anandamide, an endogenous cannabinomimetic substance, modulates rat brain protein kinase C in vitro (literal)

Abstract

Anandamide (AnNH, N-arachidonoyl-ethanolamine) has been recently proposed as the endogenous ligand for mammalian brain cannabinoid receptor. Non-cannabinoid receptor-mediated, intracellular actions have been also found for this novel mediator. Here we present evidence for the modulation by anandamide of rat brain protein kinase C (PKC) activity in vitro. The ethanolamide of arachidonic acid (AA) was more active than the free acid in increasing phosphatidylserine (PS)-induced PKC activation (EC50 = 40 microM), but inhibited dioleylglycerol-induced potentiation of both Ca(2+)- and Ca2+/PS-induced PKC activation (IC50 = 8 microM and 30 microM, respectively). A dual modulatory action of anandamide on PKC, exerted by binding to the diacylglycerol regulatory site, is hypothesized in rat brain. (literal)

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