The novel endogenous cannabinoid 2-arachidonoylglycerol is inactivated by neuronal- and basophil-like cells: connections with anandamide (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• The novel endogenous cannabinoid 2-arachidonoylglycerol is inactivated by neuronal- and basophil-like cells: connections with anandamide (Articolo in rivista) (literal)

Anno

• 1998-01-01T00:00:00+01:00 (literal)

Alternative label

• Di Marzo V, Bisogno T, Sugiura T, Melck D, De Petrocellis L. (1998)
  The novel endogenous cannabinoid 2-arachidonoylglycerol is inactivated by neuronal- and basophil-like cells: connections with anandamide
  in Biochemical journal (Lond., 1984); PORTLAND PRESS,, LONDON W1N 3AJ ENGLAND (Regno Unito)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Di Marzo V, Bisogno T, Sugiura T, Melck D, De Petrocellis L. (literal)

Pagina inizio

• 15 (literal)

Pagina fine

• 19 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 331 (literal)

Rivista

• Biochemical journal (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. CNR, Ist Chim Mol Interesse Biol, I-80072 Arco Felice, Napoli, Italy 2. Teikyo Univ, Fac Pharmaceut Sci, Kanagawa, Japan 3. CNR, Ist Cibernet, I-80072 Arco Felice, Napoli, Italy 4. CNR, Natl Inst Chem Biol Syst, I-00185 Rome, Italy (literal)

Titolo

• The novel endogenous cannabinoid 2-arachidonoylglycerol is inactivated by neuronal- and basophil-like cells: connections with anandamide (literal)

Abstract

• The novel endogenous cannabinoid 2-arachidonoylglycerol (2-AG) was rapidly inactivated by intact rat basophilic leukaemia (RBL-2H3) and mouse neuroblastoma (N18TG2) cells through diffusion/hydrolysis/reacylation processes. The hydrolysis of 2-AG was inhibited by typical esterase inhibitors and by more specific blockers of 'fatty acid amide hydrolase' (FAAH), the enzyme catalysing the hydrolysis of the other 'endocannabinoid', anandamide (AEA). No evidence for a facilitated-diffusion process was found. A 2-AG-hydrolysing activity was detected in homogenates from both cell lines, with the highest levels in membrane fractions. It exhibited an optimal pH at 10, and recognized both 2- and 1(3)- isomers of monoarachidonoylglycerol with similar efficiencies. The apparent K-m and V-max valuer for [H-3]2-AG hydrolysis were 91 mu M and 29 mu M and 2.4 and 1.8 nmol.min(-1).mg of protein(-1) respectively in N18TG2 and RBL-2H3 cells. [H-3]2-AG hydrolysis was inhibited by Cu2+, Zn2+ and p-hydroxymercuribenzoate, and by 2- or 1(3)-monolinoleoyl- and -linolenoyl-glycerols, but not by the oleoyl, palmitoyl and myristoyl congeners. Purified fractions from solubilized membrane proteins catalysed, at pH 9.5, the hydrolysis of 2-AG as well as AEA. Accordingly, AEA as well as FAAH inhibitors, including arachidonoyltrifluoromethyl ketone (ATFMK), blocked [H-3]2-AG hydrolysis by N18TG2 and RBL-2H3 membranes, whereas 2-AG inhibited [C-14]AEA hydrolysis. FAAH blockade by ATFMK preserved from inactivation the 2-AG synthesized ne novo by intact N18TG2 cells stimulated with ionomycin. These data suggest that FAAH may be one of the enzymes deputed to the physiological inactivation of 2-AG, and create intriguing possibilities for the cross-regulation of 2-AG and AEA levels. (literal)

Editore

• PORTLAND PRESS, (Editore)

Prodotto di

• Istituto di chimica biomolecolare (ICB) (Istituto)
• Istituto di cibernetica \"Edoardo Caianiello\" (ISASI) (Istituto)

Autore CNR

• DOMINIQUE JULIETTE MELCK (Unità di personale interno)
• LUCIANO DE PETROCELLIS (Persona)
• TIZIANA BISOGNO (Unità di personale interno)
• VINCENZO DI MARZO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "The novel endogenous cannabinoid 2-arachidonoylglycerol is inactivated by neuronal- and basophil-like cells: connections with anandamide" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• TIZIANA BISOGNO (Unità di personale interno)
• DOMINIQUE JULIETTE MELCK (Unità di personale interno)
• VINCENZO DI MARZO (Unità di personale interno)
• LUCIANO DE PETROCELLIS (Persona)

Prodotto

• Istituto di cibernetica \"Edoardo Caianiello\" (ISASI) (Istituto)
• Istituto di chimica biomolecolare (ICB) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biochemical journal (Rivista)

Editore di

• PORTLAND PRESS, (Editore)

Insieme di parole chiave di

• Keywords of "The novel endogenous cannabinoid 2-arachidonoylglycerol is inactivated by neuronal- and basophil-like cells: connections with anandamide" (Insieme di parole chiave)