http://www.cnr.it/ontology/cnr/individuo/prodotto/ID19696
Structure of S35C flavodoxin mutant from Desulfovibrio vulgaris in the semiquinone state. (Articolo in rivista)
- Type
- Label
- Structure of S35C flavodoxin mutant from Desulfovibrio vulgaris in the semiquinone state. (Articolo in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
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- Artali R.; Marchini N.; Meneghetti F.; Cavazzini D.; Cassetta A.; Sassone C. (literal)
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- ISI Web of Science (WOS) (literal)
- Scopu (literal)
- PubMe (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Artali R. -Institute of Pharmaceutical Chemistry, University of Milano
Marchini N. -Institute of Pharmaceutical Chemistry, University of Milano
Meneghetti F. -Institute of Pharmaceutical Chemistry, University of Milano
Cavazzini D. -Department of Biochemistry and Molecular Biology, University of Parma
Cassetta A. -IC-CNR, Trieste
Sassone C. -Department of Human and Animal Biology, University of Torino (literal)
- Titolo
- Structure of S35C flavodoxin mutant from Desulfovibrio vulgaris in the semiquinone state. (literal)
- Abstract
- The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein. (literal)
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