http://www.cnr.it/ontology/cnr/individuo/prodotto/ID196913
Evidence for an endocannabinoid system in the central nervous system of the leech Hirudo medicinalis (Articolo in rivista)
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- Evidence for an endocannabinoid system in the central nervous system of the leech Hirudo medicinalis (Articolo in rivista) (literal)
- Anno
- 2001-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/S0169-328X(00)00290-4 (literal)
- Alternative label
Matias I, Bisogno T, Melck D, Vandenbulcke F, Verger-Bocquet M, De Petrocellis L, Sergheraert C, Breton C, Di Marzo V, Salzet M. (2001)
Evidence for an endocannabinoid system in the central nervous system of the leech Hirudo medicinalis
in Molecular brain research; ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, AMSTERDAM (Paesi Bassi)
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- Matias I, Bisogno T, Melck D, Vandenbulcke F, Verger-Bocquet M, De Petrocellis L, Sergheraert C, Breton C, Di Marzo V, Salzet M. (literal)
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- 1. Univ Sci & Tech Lille Flandres Artois, Lab Endocrinol & Immunite Annelides, CNRS, UPRESA 8017,SN3, F-59655 Villeneuve Dascq, France
2. CNR, Ist Chim Mol Interesse Biol, Endocannabinoid Res Grp, I-80072 Arco, Napoli, Italy
3. CNR, Ist Cibernetica, Endocannabinoid Res Grp, I-80072 Arco, Napoli, Italy
4. Inst Biol Lille, CNRS, UMR 8525, Lab Synth Struct & Fonct Biomol, F-59021 Lille, France (literal)
- Titolo
- Evidence for an endocannabinoid system in the central nervous system of the leech Hirudo medicinalis (literal)
- Abstract
- In invertebrates, like Hydra and sea urchins, evidence for a functional cannabinoid system was described. The partial characterization of a putative CB1 cannabinoid receptor in the leech Hirudo medicinalis led us to investigate the presence of a complete endogenous cannabinoid system in this organism. By using gas chromatography-mass spectrometry, we demonstrate the presence of the endocannabinoids anandamide (N-arachidonoylethanolamine, 21.5+/-0.7 pmol/g) and 2-arachidonoyl-glycerol (147.4+/-42.7 pmol/g), and of the biosynthetic precursor of anandamide, N-arachidonylphosphatidyl-ethanolamine (16.5+/-3.3 pmol/g), in the leech central nervous system (CNS). Anandamide-related molecules such as N-palmitoylethanolamine (32.4+/-1.6 pmol/g) and N-linolenoylethanolamine (5.8 pmol/g) were also detected. We also found an anandamide amidase activity in the leech CNS cytosolic fraction with a maximal activity at pH 7 and little sensitivity to typical fatty acid amide hydrolase (FAAH) inhibitors. Using an antiserum directed against the amidase signature sequence, we focused on the identification and the localization of the leech amidase. Firstly, leech nervous system protein extract was subjected to Western blot analysis, which showed three immunoreactive bands at ca. similar to 42, similar to 46 and similar to 66 kDa. The former and latter bands were very faint and were also detected in whole homogenates from the coelenterate Hydra vulgaris, where the presence of CB1-like receptors, endocannabinoids and a FAAH-like activity was reported previously. Secondly, amidase immunocytochemical detection revealed numerous immunoreactive neurons in the CNS of three species of leeches. In addition, we observed that leech amidase-like immunoreactivity matches to a certain extent with CB1-like immunoreactivity. Finally, we also found that stimulation by anandamide of this receptor leads, as in mammals, to inhibition of cAMP formation, although this effect appeared to be occurring through the previously described anandamide-induced and CB1-mediated activation of nitric oxide release. Taken together, these results suggest the existence of a complete and functional cannabinoid system in leeches. (literal)
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