Involvement of Polyamine Oxidase in Wound Healing (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Involvement of Polyamine Oxidase in Wound Healing (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1104/pp.107.108902 (literal)

Alternative label

• Angelini Riccardo,Tisi Alessandra , Rea Giuseppina, Chen Martha M. , Botta Maurizio , Federico Rodolfo and Cona Alessandra (2008)
  Involvement of Polyamine Oxidase in Wound Healing
  in Plant physiology (Bethesda)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Angelini Riccardo,Tisi Alessandra , Rea Giuseppina, Chen Martha M. , Botta Maurizio , Federico Rodolfo and Cona Alessandra (literal)

Pagina inizio

• 162 (literal)

Pagina fine

• 177 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.plantphysiol.org (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 146 (literal)

Rivista

• Plant physiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• published on november 9 2007 as DOI:10.1104/pp.107.108902 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 16 (literal)

Note

• Scopu (literal)
• ISI Web of Science (WOS) (literal)
• Google Scholar (literal)
• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dipartimento di Biologia, Università Roma Tre, Viale G. Marconi 446, 00146 Rome, Italy (R.A., A.T., M.M.C., R.F., A.C.); Istituto di Cristallografia, Consiglio Nazionale delle Ricerche, Monterotondo, 00016 Rome, Italy (G.R.); and Dipartimento Farmaco Chimico Tecnologico, Universita` degli Studi di Siena, Via A. Moro, 53100 Siena, Italy (M.B.) (literal)

Titolo

• Involvement of Polyamine Oxidase in Wound Healing (literal)

Abstract

• Hydrogen peroxide (H2O2) is involved in plant defense responses that follow mechanical damage, such as those that occur during herbivore or insect attacks, as well as pathogen attack. H2O2 accumulation is induced during wound healing processes as well as by treatment with the wound signal jasmonic acid. Plant polyamine oxidases (PAOs) are H2O2 producing enzymes supposedly involved in cell wall differentiation processes and defense responses. Maize (Zea mays) PAO (ZmPAO) is a developmentally regulated flavoprotein abundant in primary and secondary cell walls of several tissues. In this study, we investigated the effect of wounding on ZmPAO gene expression in the outer tissues of the maize mesocotyl and provide evidence that ZmPAO enzyme activity, protein, and mRNA levels increased in response to wounding as well as jasmonic acid treatment. Histochemically detected ZmPAO activity especially intensified in the epidermis and in the wound periderm, suggesting a tissue-specific involvement of ZmPAO in wound healing. The role played by ZmPAO-derived (HO2)-O-2 production in peroxidase- mediated wall stiffening events was further investigated by exploiting the in vivo use of N-prenylagmatine (G3), a selective and powerful ZmPAO inhibitor, representing a reliable diagnostic tool in discriminating ZmPAO-mediated H2O2 production from that generated by peroxidase, oxalate oxidase, or by NADPH oxidase activity. Here, we demonstrate that G3 inhibits wound-induced H2O2 production and strongly reduces lignin and suberin polyphenolic domain deposition along the wound, while it is ineffective in inhibiting the deposition of suberin aliphatic domain. Moreover, ZmPAO ectopic expression in the cell wall of transgenic tobacco (Nicotiana tabacum) plants strongly enhanced lignosuberization along the wound periderm, providing evidence for a causal relationship between PAO and peroxidase-mediated events during wound healing. developmentally regulated flavoprotein abundant in primary and secondary cell walls of several tissues. In this study, we investigated the effect of wounding on ZmPAO gene expression in the outer tissues of the maize mesocotyl and provide evidence that ZmPAO enzyme activity, protein, and mRNA levels increased in response to wounding as well as jasmonic acid treatment. Histochemically detected ZmPAO activity especially intensified in the epidermis and in the wound periderm, suggesting a tissue-specific involvement of ZmPAO in wound healing. The role played by ZmPAO-derived H2O2 production in peroxidase-mediated wall stiffening events was further investigated by exploiting the in vivo use of N-prenylagmatine (G3), a selective and powerful ZmPAO inhibitor, representing a reliable diagnostic tool in discriminating ZmPAO-mediated H2O2 production from that generated by peroxidase, oxalate oxidase, or by NADPH oxidase activity. Here, we demonstrate that G3 inhibits wound-induced H2O2 production and strongly reduces lignin and suberin polyphenolic domain deposition along the wound, while it is ineffective in inhibiting the deposition of suberin aliphatic domain. Moreover, ZmPAO ectopic expression in the cell wall of transgenic tobacco (Nicotiana tabacum) plants strongly enhanced lignosuberization along the wound periderm, providing evidence for a causal relationship between PAO and peroxidase-mediated events during wound healing. (literal)

Prodotto di

• NUTRACEUTICI IN APPLICAZIONI SPAZIALI E TRASFERIMENTO TECNOLOGICO A TERRA (AG.P05.014.001) (Modulo)
• Institute of Crystallography (IC) (Istituto)

Autore CNR

• GIUSEPPINA REA (Unità di personale interno)

Incoming links:

Prodotto

• Institute of Crystallography (IC) (Istituto)
• NUTRACEUTICI IN APPLICAZIONI SPAZIALI E TRASFERIMENTO TECNOLOGICO A TERRA (AG.P05.014.001) (Modulo)

Autore CNR di

• GIUSEPPINA REA (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Plant physiology (Rivista)