Separation of multicomponent mixtures of 2,4-dinitrophenyl labelled amino acids and their enantiomers by capillary zone electrophoresis (Articolo in rivista)

Type
Label
  • Separation of multicomponent mixtures of 2,4-dinitrophenyl labelled amino acids and their enantiomers by capillary zone electrophoresis (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/1522-2683(200102)22:3<470::AID-ELPS470>3.0.CO;2-G (literal)
Alternative label
  • Mikus P, Kaniansky D, Fanali S (2001)
    Separation of multicomponent mixtures of 2,4-dinitrophenyl labelled amino acids and their enantiomers by capillary zone electrophoresis
    in Electrophoresis (Weinh., Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mikus P, Kaniansky D, Fanali S (literal)
Pagina inizio
  • 470 (literal)
Pagina fine
  • 477 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 22 (literal)
Rivista
Note
  • Scopus (literal)
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • University of Bratislava; University of Bratislava; IMC-CNR (literal)
Titolo
  • Separation of multicomponent mixtures of 2,4-dinitrophenyl labelled amino acids and their enantiomers by capillary zone electrophoresis (literal)
Abstract
  • The use of capillary zone electrophoresis (CZE) for the separation of a group of 33 2,4-dinitrophenyl labeled amino acids (DNP-AA), including DNP-AA racemates, DNP-L-AA enantiomers and achiral DNP-AAs, was investigated. ?-, ?- and ?-cyclodextrins (CDs) and their derivatives (hydroxypropyl derivatives of ?-, ?- and ?-CDs, polymeric ?-CD and 6A-methylamino-?-cyclodextrin (MA-?-CD)) served as complexing agents and chiral selectors in this investigation. Although native ?- and ?-CDs and their derivatives influenced the effective mobilities of the studied DNP-AAs in different ways, they generally failed to resolve enantiomers of the individual DNP-AAs. On the other hand, ?-CD and all of its derivatives were found to be effective in this respect. Of these, the best results were achieved with a positively charged MA-?-CD and this chiral selector resolved enantiomers of ten DNP-AA racemates available for this study. However, a complete resolution of these enantiomers in one CZE run required that the effect of the chiral selector be complemented by complexing effects of polyvinyl pyrrolidone (PVP) or ?-CD. Complexing and chiral recognition capabilities of MA-?-CD combined with complexing effects of ?-CD and PVP provided separating conditions suitable for the CZE separations of multicomponent mixtures of DNP-AAs with preserved resolutions of the enantiomers. For example, a mixture consisting of 43 DNP-AA constituents was resolved using an MA-?-CD/?-CD combination with three peak overlaps. (literal)
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