Role of the major histocompatibility complex (MHC) class II molecules mediated signalling in melanoma cells (Contributo in atti di convegno)

Type
Label
  • Role of the major histocompatibility complex (MHC) class II molecules mediated signalling in melanoma cells (Contributo in atti di convegno) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Alternative label
  • Francesca Costantini, Maria Assunta Costa and Giovanna Barbieri (2012)
    Role of the major histocompatibility complex (MHC) class II molecules mediated signalling in melanoma cells
    in 22nd biennal congress of EACR 22, Barcellona, Spagna, 7-10 luglio
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Francesca Costantini, Maria Assunta Costa and Giovanna Barbieri (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume
  • proceeding book (literal)
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  • 48 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 5 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Biomedicina e Immunologia Molecolare \"Alberto Monroy\", Consiglio Nazionale delle Ricerche (CNR), Palermo, Italy (literal)
Titolo
  • Role of the major histocompatibility complex (MHC) class II molecules mediated signalling in melanoma cells (literal)
Abstract
  • Background: Cellular processes such as cell survival, growth, migration and gene expression are modulated by the interaction between cells and their microenvironment. Cell adhesion molecules (CAMs) that include cadherins, integrins and selectins, are the main receptors that facilitate the cells anchorage to the extracellular matrix and the cell-cell adhesion. The alteration of CAMs expression is related to metastatic progression of melanoma through the disruption of normal interactions between cells and cells with the matrix. Furthermore, the progression of melanoma is also associated to the constitutive expression of Major Histocompatibility Complex (MHC) class II molecules in melanoma cells. Indeed, the MHC class II molecules in addition to their well-established role as antigen-presenting molecules are also important signalling receptors. The aim of our work is to understand the impact of signalling mediated by MHC class II molecules in CAMs receptors expression and therefore in melanoma progression to an invasive-metastatic state. Material and methods: Two MHC class II constitutive expressing human melanoma cell lines (A375 and HT144) with marked heterogeneity respect to invasion potential were stimulated for 24h, 48h and 72h with a specific anti-HLA-DR mAb (L243) that mimics the TCR interaction with class II molecules or left unstimulated. Therefore, the CAMs and HLA-DR? expression profile was analysed in total cell extracts. Exosomes secreted by stimulated and unstimulated melanoma cells were purified and analysed by western blot. Results and discussion: Within the hypothesis that MHC class II mediated signalling in constitutive expressing melanoma cells might have a role in melanoma progression, we compared in stimulated and unstimulated A375 and HT144 cell lines the expression levels of class II and CAMs receptors as well as the exosomes secreted. The results obtained showed an in vitro heterogeneity with respect to CAMs and HLA-DR? proteins expression in stimulated and unstimulated A375 and HT144 cells. Conclusions: Our results provide some interesting information about the consequences of TCRs and MHC class II association that is mimed here by a specific anti-MHC class II antibody. In particular, this study helps to elucidate how the class II dependent signalling could affect in melanoma the extracellular matrix adhesion, the cell-to-cell interaction, the exosomes role on microenvironment and therefore the metastatic dissemination of melanoma. (literal)
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