http://www.cnr.it/ontology/cnr/individuo/prodotto/ID195521

Human lymphocytes cultured in 3-D bioreactors: Influence of configuration (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Human lymphocytes cultured in 3-D bioreactors: Influence of configuration (Articolo in rivista) (literal)

Anno

2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.biomaterials.2012.07.065 (literal)

Alternative label

Efrem Curcio, Antonella Piscioneri, Simona Salerno, Franco Tasselli, Sabrina Morelli, Enrico Drioli, Loredana De Bartolo (2012)
Human lymphocytes cultured in 3-D bioreactors: Influence of configuration
in Biomaterials
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Efrem Curcio, Antonella Piscioneri, Simona Salerno, Franco Tasselli, Sabrina Morelli, Enrico Drioli, Loredana De Bartolo (literal)

Pagina inizio

8296 (literal)

Pagina fine

8303 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

33 (literal)

Rivista

Biomaterials (Rivista)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

a Department of Chemical Engineering and Materials, University of Calabria, via P. Bucci cubo 45/A, I-87030 Rende (CS), Italy b Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci cubo 17/C, I-87030 Rende (CS), Italy c Hanyang University, WCU Energy Engineering Department, Seoul, South Korea (literal)

Titolo

Human lymphocytes cultured in 3-D bioreactors: Influence of configuration (literal)

Abstract

Peripheral blood lymphocytes isolated from healthy human donors' buffy coat were cultured in membrane bio-reactors (MBR) designed in two different configurations: a conventional hollow-fiber (HF) bundle of modified polyetheretherketone (PEEK-WC) arranged in parallel, and a cross-assembled PEEK-WC and polyethersulfone (PES) HF membranes having different structural properties. Both bioreactors were experimentally compared in terms of metabolic activity of cultured cells, monitored over 8 days with respect to glucose uptake rate (GUR) and lactate production rate (LPR), and mathematically modelled by Computational Fluid Dynamics (CFD) method in order to investigate the impact of geometrical configuration and transport properties of biomaterials. The almost uniform trend of GUR from day 2 to day 7 (average of 0.0497 ? 0.0076 ng/h cell) and the lowLPR (that decreased from an initial value of 2.92 ? 0.0055 pg/h cell to practically zero at day 8) provided evidence for superior performance of crossed-HFMBR in reproducing an optimal in vitro physiological environment with quite uniform concentration distribution of species in the extracellular space of the bioreactor and able to maintain lymphocyte viability and functions. The crossed HFMBR also resulted in an enhanced production of interleukin IL-2 over 8 days (average of 0.995?0.25 pg/h/ Mcell) and IL-10 in the first 3 days (average of 6.46 ? 0.28 pg/h/Mcell) which were up to one order of magnitude higher with respect to values measured in the parallel configuration. (literal)

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