Luminescent Conjugates between Dinuclear Rhenium Complexes and Peptide Nucleic Acids (PNA): Synthesis, Photophysical Characterization, and Cell Uptake (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Luminescent Conjugates between Dinuclear Rhenium Complexes and Peptide Nucleic Acids (PNA): Synthesis, Photophysical Characterization, and Cell Uptake (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1021/om3004515 (literal)

Alternative label

• Mari, C; Panigati, M; D'Alfonso, L; Zanoni, I; Donghi, D;Sironi, L; Collini, M; Maiorana, S; Baldoli, C; D'Alfonso, G; Licandro, E. (2012)
  Luminescent Conjugates between Dinuclear Rhenium Complexes and Peptide Nucleic Acids (PNA): Synthesis, Photophysical Characterization, and Cell Uptake
  in Organometallics; ACS, American chemical society, Washington, DC (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Mari, C; Panigati, M; D'Alfonso, L; Zanoni, I; Donghi, D;Sironi, L; Collini, M; Maiorana, S; Baldoli, C; D'Alfonso, G; Licandro, E. (literal)

Pagina inizio

• 5918 (literal)

Pagina fine

• 5928 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 31 (literal)

Rivista

• Organometallics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 16 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Università di Milano, Dipartimento Chimica, I-20133 Milan, Italy Univ Milano Bicocca, I-20126 Milan, Italy (literal)

Titolo

• Luminescent Conjugates between Dinuclear Rhenium Complexes and Peptide Nucleic Acids (PNA): Synthesis, Photophysical Characterization, and Cell Uptake (literal)

Abstract

• Different PNA decamers have been appended to a luminescent [Re-2(mu-Cl)(2)(CO)(6)(mu-1,2-diazine)] complex, to obtain conjugates suitable for cellular imaging. The new compounds can be dissolved in water in the presence of an amount of DMSO as small as 0.4-0.6 mol %. The conjugation with PNA did not perturb the photoluminescence behavior of the organometallic fragment: emission from (MLCT)-M-3 excited states, centered at ca. 610 nm, was observed, with satisfactory photoluminescence quantum yields (Phi = ca. 0.01, in aerated water). Moreover, the emission could be stimulated also by two-photon excitation. Experiments of cell uptake, performed with different cell lines and under different experimental conditions, showed that the nature of the PNA oligomer strongly affects the biointeraction, while no major differences were observed among the cell lines investigated. The Re-PNA conjugates containing neutral PNA decamers (either homothymine or a standard sequence of the four nucleobases) showed a marked tendency to concentrate in the nuclear region, whereas nucleus penetration was more difficult for the free dirhenium complex. Staining of nucleus and cytoplasm with different colors was generally observed. No nucleus penetration was instead observed for a water-soluble Re-PNA homothymine decamer end-capped with four lysine residues, which localized in endosome-like compartments. (literal)

Editore

• ACS, American chemical society (Editore)

Prodotto di

• Istituto di scienze e tecnologie molecolari (ISTM) (Istituto)
• Sintesi e caratterizzazione di molecole organiche e complessi metallorganici con specifiche proprietà ottiche (PM.P04.012.002) (Modulo)

Autore CNR

• CLARA BALDOLI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Luminescent Conjugates between Dinuclear Rhenium Complexes and Peptide Nucleic Acids (PNA): Synthesis, Photophysical Characterization, and Cell Uptake" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di scienze e tecnologie molecolari (ISTM) (Istituto)
• Sintesi e caratterizzazione di molecole organiche e complessi metallorganici con specifiche proprietà ottiche (PM.P04.012.002) (Modulo)

Autore CNR di

• CLARA BALDOLI (Unità di personale interno)

Editore di

• ACS, American chemical society (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Organometallics (Rivista)

Insieme di parole chiave di

• Parole chiave di "Luminescent Conjugates between Dinuclear Rhenium Complexes and Peptide Nucleic Acids (PNA): Synthesis, Photophysical Characterization, and Cell Uptake" (Insieme di parole chiave)