http://www.cnr.it/ontology/cnr/individuo/prodotto/ID194922
Molecular characterization, phylogenetic comparison and serological relationship of the Imp protein of several 'Candidatus Phytoplasma aurantifolia' strains (Articolo in rivista)
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- Molecular characterization, phylogenetic comparison and serological relationship of the Imp protein of several 'Candidatus Phytoplasma aurantifolia' strains (Articolo in rivista) (literal)
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- 2013-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1111/j.1365-3059.2012.02662.x (literal)
- Alternative label
Siampour M., Izadpanah K., Galetto L., Salehic M., Marzachì C. (2013)
Molecular characterization, phylogenetic comparison and serological relationship of the Imp protein of several 'Candidatus Phytoplasma aurantifolia' strains
in Plant pathology (Online); Blackwell, Oxford (Regno Unito)
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- Siampour M., Izadpanah K., Galetto L., Salehic M., Marzachì C. (literal)
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- S.M. & I.K.: PLANT VIROLOGY RESEARCH CENTER, COLLEGE OF AGRICULTURE, SHIRAZ UNIVERSITY, 33111-71441 SHIRAZ, IRAN
S.M.:PLANT PROTECTION RESEARCH, DEPARTMENT, AGRICULTURAL AND NATURAL RESOURCES RESEARCH CENTER OF FARS, 73415-53111 ZARGHAN, IRAN
G.L. & M.C.: IVV - CNR (literal)
- Titolo
- Molecular characterization, phylogenetic comparison and serological relationship of the Imp protein of several 'Candidatus Phytoplasma aurantifolia' strains (literal)
- Abstract
- The immunodominant membrane protein Imp of several phytoplasmas within the 'Candidatus Phytoplasma aurantifolia'
(16Sr-II) group was investigated. Eighteen isolates from Iran (11), East Asia (5), Africa (1) and Australia (1) clustered into
three phylogenetic subgroups (A, B and C) based on the 16S rDNA and imp genes, regardless of geographic origin. The imp
gene sequences were variable, with more non-synonymous than synonymous mutations (68 vs 20, respectively), even though
many of the non-synonymous ones (75%) produced conservative amino acid replacements. Eight codon sites on the extracellular
region of the protein were under positive selection, with most of them (75%) coding for non-conservative amino
acid substitutions. Full-length (21 kDa) and truncated (16 kDa) Imp proteins of two economically important Iranian phytoplasmas
[lime witches' broom (LWB) and alfalfa witches' broom (AlWB-F)] were expressed as His-tagged recombinant
proteins in Escherichia coli. An antiserum raised against full-length recombinant LWB Imp reacted in western blots with
membrane proteins extracted from LWB-infected periwinkle and lime, indicating that Imp (19 kDa) is expressed in infected
plants and is a membrane-associated protein. The same polyclonal antibody also detected native Imp in proteins from periwinkles
infected by phytoplasmas closely related to LWB (subgroup C) only, confirming phylogenetic clustering based on
16S rDNA and imp genes. Imp proteins of LWB and AlWB-F isolates were also recognized by an antiserum raised against
an enriched preparation of AlWB-F phytoplasma cells, demonstrating the antigenic properties of this protein. (literal)
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