http://www.cnr.it/ontology/cnr/individuo/prodotto/ID194846
Inhibition of Choroidal and Corneal Pathologic Neovascularization by Plgf1-de Gene Transfer. (Articolo in rivista)
- Type
- Label
- Inhibition of Choroidal and Corneal Pathologic Neovascularization by Plgf1-de Gene Transfer. (Articolo in rivista) (literal)
- Anno
- 2012-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1167/iovs.12-10658. (literal)
- Alternative label
Tarallo V, Bogdanovich S, Hirano Y, Tudisco L, Zentilin L, Giacca M, Ambati J, De Falco S. (2012)
Inhibition of Choroidal and Corneal Pathologic Neovascularization by Plgf1-de Gene Transfer.
in Investigative ophthalmology & visual science
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Tarallo V, Bogdanovich S, Hirano Y, Tudisco L, Zentilin L, Giacca M, Ambati J, De Falco S. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Angiogenesis Lab, Institute of Genetics and Biophysics, CNR, Naples, Italy;
Department of Ophthalmology & Visual Sciences, University of Kentucky,Lexington, KY, USA;
Molecular Medicine Laboratory, ICGEB, Trieste, Italy. (literal)
- Titolo
- Inhibition of Choroidal and Corneal Pathologic Neovascularization by Plgf1-de Gene Transfer. (literal)
- Abstract
- Ocular neovascularization (NV), the primary cause of blindness, typically is treated via inhibition of VEGF-A activity. However, besides VEGF-A, other proteins of the same family, including VEGF-B and placental growth factor (PlGF, all together VEGFs), have a crucial role in the angiogenesis process. PlGF and VEGF, which form heterodimers if co-expressed, both are required for pathologic angiogenesis. We generated a PlGF1 variant, named PlGF1-DE, which is unable to bind and activate VEGFR-1, but retains the ability to form heterodimer. PlGF1-DE acts as dominant negative of VEGF-A and PlGF1wt through heterodimerization mechanism. The purpose of our study was to explore the therapeutic potential of Plgf1-de gene in choroid and cornea NV context.
METHODS:
In the model of laser-induced choroidal neovascularization (CNV), Plgf1-de gene, and as control Plgf1wt, LacZ, or gfp genes, were delivered using adeno-associated virus (AAV) vector by subretinal injection 14 days before the injury. After 7 days CNV volume was assessed. Corneal NV was induced by scrape or suture procedures. Expression vectors for PlGF1wt or PlGF1-DE, and as control the empty vector pCDNA3, were injected in the mouse cornea after the vascularization insults. NV was evaluated with CD31 and LYVE-1 immunostaining.
RESULTS:
The expression of Plgf1-de induced significant inhibition of choroidal and corneal NV by reducing VEGF-A homodimer production. Conversely, the delivery of Plgf1wt, despite induced similar reduction of VEGF-A production, did not affect NV.
CONCLUSIONS:
Plgf1-de gene is a new therapeutic tool for the inhibition of VEGFs driven ocular NV. (literal)
- Prodotto di
- Autore CNR
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