http://www.cnr.it/ontology/cnr/individuo/prodotto/ID194736
An aryleneethynylene fluorophore for cell membrane staining (Articolo in rivista)
- Type
- Label
- An aryleneethynylene fluorophore for cell membrane staining (Articolo in rivista) (literal)
- Anno
- 2012-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.bbamem.2012.06.011 (literal)
- Alternative label
Cardone, Antonio; Lopez, Francesco; Affortunato, Francesco; Busco, Giovanni; Hofer, Aldebaran M.;
Mallamaci, Rosanna; Martinelli, Carmela; Colella, Matilde; Farinola, Gianluca M. (2012)
An aryleneethynylene fluorophore for cell membrane staining
in Biochimica et biophysica acta. Biomembranes; Elsevier Ltd, Oxford (Regno Unito)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Cardone, Antonio; Lopez, Francesco; Affortunato, Francesco; Busco, Giovanni; Hofer, Aldebaran M.;
Mallamaci, Rosanna; Martinelli, Carmela; Colella, Matilde; Farinola, Gianluca M. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
- Note
- ISI Web of Science (WOS) (literal)
- Scopus (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Consiglio Nazionale delle Ricerche, Istituto di Chimica dei Composti OrganoMetallici, UOS di Bari, Via Orabona, 4-70125 Bari, Italy
Dipartimento di Agricoltura, Ambiente ed Alimenti (DIAAA) and CSGI, Università degli Studi del Molise, I-86100 Campobasso, Italy
Dipartimento di Bioscienze, Biotecnologie e Scienze Farmacologiche, Università degli Studi di Bari \"Aldo Moro\", Via Orabona, 4-70125 Bari, Italy
VA Boston Healthcare System and the Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, 1400 VFW Parkway, West Roxbury, MA 02132, USA
Dipartimento di Chimica, Università degli Studi di Bari \"Aldo Moro\", Via Orabona, 4-70125 Bari Italy (literal)
- Titolo
- An aryleneethynylene fluorophore for cell membrane staining (literal)
- Abstract
- The use of an amphiphilic aryleneethynylene fluorophore as a plasma membrane marker in fixed and living
mammalian cells and liposome model systems is demonstrated. We show here that the optical properties of
the novel dye are almost independent on pH, in the range 5.0-8.0. Spectroscopic characterization performed
on unilamellar liposomes ascertained that the fluorescence intensity of the aryleneethynylene fluorophore
greatly increases after incorporation in lipidic membranes. Experiments performed on different mammalian
cells demonstrated that the novel membrane marker exhibits fast staining and a good photostability that
make it a suitable tool for live cell imaging. Importantly, the aryleneethynylene fluorophore was also
shown to be a fast and reliable blue membrane marker in classical multicolor immunofluorescence experiments.
This study adds new important findings to the recent exploitation of the wide class of
aryleneethynylene molecules as luminescent markers for biological investigations. (literal)
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- Autore CNR
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