Cell self-patterning on uniform PDMS-surfaces with controlled mechanical cues (Articolo in rivista)

Type
Label
  • Cell self-patterning on uniform PDMS-surfaces with controlled mechanical cues (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1039/c2ib00116k (literal)
Alternative label
  • IE Palamà; S. D'Amone, A.M.L Colucci, M. Biasiucci, G Gigli (2012)
    Cell self-patterning on uniform PDMS-surfaces with controlled mechanical cues
    in Integrative biology (Camb., Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • IE Palamà; S. D'Amone, A.M.L Colucci, M. Biasiucci, G Gigli (literal)
Pagina inizio
  • 228 (literal)
Pagina fine
  • 236 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 4 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. Inst Nanosci CNR, NNL, I-73100 Lecce, Italy 2. Univ Salento, Scuola Super ISUFI, I-73100 Lecce, Italy 3. Univ Salento, Vito Fazzi Hosp, Hematol & Clin Prote Unit, I-73100 Lecce, Italy 4. Univ Roma La Sapienza, Dipartimento Fis, I-00133 Rome, Italy 5. Univ Roma Tor Vergata, Dept Elect Engn, I-00133 Rome, Italy 6. Univ Salento, Dipartimento Ingn Innovaz, I-73100 Lecce, Italy 7. Italian Inst Technol IIT, Ctr Biomol Nanotechnol CNB, Arnesano, Le, Italy (literal)
Titolo
  • Cell self-patterning on uniform PDMS-surfaces with controlled mechanical cues (literal)
Abstract
  • The exploitation of cell-instructive scaffolds with uniform physical/chemical surfaces and controlled stiffness will be greatly useful in tissue engineering applications to resemble the extracellular matrix (ECM) or topographical appearance of native tissues. We herein describe a versatile and straightforward method to assemble a polydimethylsiloxane (PDMS)-composite structure in which a uniformly laminin-coated membrane is placed on top of a micropatterned substrate that applies a stiffness gradient. This 'double-sheet' structure provides soft or stiff microdomains that guide the self-patterning of different cell types [e.g. chronic myeloid leukemia (KU812), cervix carcinoma (HeLa), NIH 3T3 and BJ], thereby stimulating their cytoskeletal remodeling. More interestingly, we used these uniform PDMS surfaces with patterned rigidity for obtaining co-cultures of tumor blood cells (KU812) and adherent fibroblasts (NIH 3T3) with spatially-controlled distribution. Thus, beyond single-cell stiffening and mechanosensing, these surfaces should also be used as simple and feasible co-culture systems for mimicking and dissecting the bidirectional interactions between blood cells and specific stromal elements of their in vivo microenvironment. (literal)
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