Biocompatible noisy nanotopographies with specific directionality for controlled anisotropic cell cultures (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Biocompatible noisy nanotopographies with specific directionality for controlled anisotropic cell cultures (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1039/c1sm06256e (literal)

Alternative label

• Meucci S; Tonazzini I; Beltram F; Cecchini M (2012)
  Biocompatible noisy nanotopographies with specific directionality for controlled anisotropic cell cultures
  in Soft matter (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Meucci S; Tonazzini I; Beltram F; Cecchini M (literal)

Pagina inizio

• 1109 (literal)

Pagina fine

• 1119 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 8 (literal)

Rivista

• Soft matter (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Scuola Normale Super Pisa, NEST, I-56127 Pisa, Italy 2. Ist Nanosci CNR, I-56127 Pisa, Italy 3. Ist Italiano Tecnol, NEST, Ctr Nanotechnol Innovat, I-56127 Pisa, Italy (literal)

Titolo

• Biocompatible noisy nanotopographies with specific directionality for controlled anisotropic cell cultures (literal)

Abstract

• All cells are exposed to extra-cellular physical stimuli determined by the details of the micro-/nano-environment within which they exist. These stimuli are present in organs and tissues where specific directional signals coexist with biotopographical noise (e.g. cellular debris, residues of apoptotic cells , protein accumulation, sclerotic plaques). Here, we present a platform for the investigation of the impact of this noise based on nanostructured plastic scaffolds with a controlled level of anisotropy. Two different types of topographical noise are introduced into fully ordered nanostructures . Starting from nanogratings, we randomly introduce nanomodifications, whose density determines the overall substrate directionality. A general quantitative definition of directionality is discussed and applied to our nanostructures . Substrate biocompatibility is assayed by culturing PC12 cells and evaluating cell viability and NGF-induced neuronal-differentiation efficiency. The suitability for high-resolution microscopy on living cells is demonstrated by visualizing focal adhesion complexes by total internal reflection fluorescence (TIRF) microscopy . Finally, we show the impact of noise in modulating focal adhesion maturation in PC12 cells upon NGF-induced neuronal differentiation. Our results indicate design rules both for biocompatible textured substrates allowing the study of cell -environment interaction in vitro and for tissue engineering applications. (literal)

Prodotto di

• Progettazione ed applicazioni di Nanobiotecnologie (MD.P06.022.002) (Modulo)

Autore CNR

• ILARIA TONAZZINI (Persona)
• Sandro MEUCCI (Unità di personale esterno)
• FABIO BELTRAM (Persona)
• MARCO CECCHINI (Unità di personale interno)

Incoming links:

Autore CNR di

• MARCO CECCHINI (Unità di personale interno)
• ILARIA TONAZZINI (Persona)
• Sandro MEUCCI (Unità di personale esterno)
• FABIO BELTRAM (Persona)

Prodotto

• Progettazione ed applicazioni di Nanobiotecnologie (MD.P06.022.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Soft matter (Rivista)