http://www.cnr.it/ontology/cnr/individuo/prodotto/ID194081

Anthracycline-dependent heat-induced transition from positive to negative supercoiled DNA (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Anthracycline-dependent heat-induced transition from positive to negative supercoiled DNA (Articolo in rivista) (literal)

Anno

2003-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1002/elps.200305388 (literal)

Alternative label

Viglasky, V ; Valle, F ; Adamcik, J; Joab, I; Podhradsky, D; Dietler, G (2003)
Anthracycline-dependent heat-induced transition from positive to negative supercoiled DNA
in Electrophoresis (Weinh., Print); WILEY-V C H VERLAG GMBH,, D-69451 WEINHEIM, (Germania)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Viglasky, V ; Valle, F ; Adamcik, J; Joab, I; Podhradsky, D; Dietler, G (literal)

Pagina inizio

1703 (literal)

Pagina fine

1711 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

24 (literal)

Rivista

Electrophoresis (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

11 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

1. Safarik Univ PJ, Fac Sci, Inst Chem, Dept Biochem, Kosice 04154, Slovakia 2. Univ Lausanne, Inst Phys Mat Condensee, Lausanne, Switzerland 3. Inst Federat Rech St Louis, Inst Mol Genet, Paris, France (literal)

Titolo

Anthracycline-dependent heat-induced transition from positive to negative supercoiled DNA (literal)

Abstract

The conformational stability of individual DNA topoisomers depends on the concentration of DNA intercalating drugs. To study the DNA-drug interaction, we used ethidium bromide (EtBr) and negative supercoiled pUC19 as a model system. The effects of two anthracyclines widely used in cancer therapy, daunorubicin (Dau) and doxorubicin (Doxo), and EtBr were compared. In spite of their different chemical structures and intercalation mode, all intercalating agents show similar effects on the conformational stability of supercoiled DNA. Our observations show that the studied intercalators have at least two main effects on the supercoiled DNA: (i) they decrease the level of negative supercoiling and, at certain concentrations, they may induce positive supercoiling in DNA; (ii) a temperature increase can cause a recovery of negative supercoiling in DNA. The conformational stability of plasmid DNA-drug complexes has been investigated by temperature gradient gel electrophoresis (TGGE). We demonstrate the suitability of TGGE for this purpose, because it offers a global view on DNA-drug complexes over a continuous range of temperature. Images of DNA plasmids adsorbed onto a substrate at different temperatures and drug concentrations were acquired by atomic force microscopy (AFM), allowing us to distinguish directly the conformation chirality assumed by the plasmid under different conditions confirming TGGE results. Our detection system allows to characterize unknown drugs and to determine their intercalating properties (literal)

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