New insight into the ochratoxin A biosynthetic pathway through deletion of a nonribosomal peptide synthetase gene in Aspergillus carbonarius. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• New insight into the ochratoxin A biosynthetic pathway through deletion of a nonribosomal peptide synthetase gene in Aspergillus carbonarius. (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1128/AEM.02508-12 (literal)

Alternative label

• Gallo A., Bruno K.S., Solfrizzo M., Perrone G., Mulè G., Visconti A., Baker S (2012)
  New insight into the ochratoxin A biosynthetic pathway through deletion of a nonribosomal peptide synthetase gene in Aspergillus carbonarius.
  in Applied and environmental microbiology (Print); American Society Of Microbiology (ASM), Washington (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Gallo A., Bruno K.S., Solfrizzo M., Perrone G., Mulè G., Visconti A., Baker S (literal)

Pagina inizio

• 8208 (literal)

Pagina fine

• 8218 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• IF 3.952 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 78 (literal)

Rivista

• Applied and environmental microbiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 23 (literal)

Note

• PubMe (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Scienze delle Produzioni Alimentari, ISPA-CNR,Bari, Italia Chemical and Biological Process Development Group, Pacific Northwest National Laboratory, Richland, Washington, USA (literal)

Titolo

• New insight into the ochratoxin A biosynthetic pathway through deletion of a nonribosomal peptide synthetase gene in Aspergillus carbonarius. (literal)

Abstract

• Ochratoxin A (OTA), a mycotoxin produced by Aspergillus and Penicillium species, is composed of a dihydroisocoumarin ring linked to phenylalanine, and its biosynthetic pathway has not yet been completely elucidated. Most of the knowledge regarding the genetic and enzymatic aspects of OTA biosynthesis has been elucidated in Penicillium species. In Aspergillus species, only pks genes involved in the initial steps of the pathway have been partially characterized. In our study, the inactivation of a gene encoding a nonribosomal peptide synthetase (NRPS) in OTA-producing A. carbonarius ITEM 5010 has eliminated the ability of this fungus to produce OTA. This is the first report on the involvement of an nrps gene product in OTA biosynthetic pathway in an Aspergillus species. The absence of OTA and ochratoxin ?, the isocoumaric derivative of OTA, and the concomitant increase of ochratoxin ?, the dechloro analog of ochratoxin ?, were observed in the liquid culture of transformed strain. The data provide the first evidence that the enzymatic step adding phenylalanine to polyketide dihydroisocoumarin precedes the chlorination step to form OTA in A. carbonarius and that ochratoxin ? is a product of hydrolysis of OTA, giving an interesting new insight into the biosynthetic pathway of the toxin. (literal)

Editore

• American Society Of Microbiology (ASM) (Editore)

Prodotto di

• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)
• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)

Autore CNR

• GIANCARLO PERRONE (Persona)
• MICHELE SOLFRIZZO (Unità di personale interno)
• ANGELO VISCONTI (Unità di personale interno)
• ANTONIA GALLO (Persona)
• GIUSEPPINA MULE' (Persona)

Insieme di parole chiave

• Keywords of "New insight into the ochratoxin A biosynthetic pathway through deletion of a nonribosomal peptide synthetase gene in Aspergillus carbonarius." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ANGELO VISCONTI (Unità di personale interno)
• GIANCARLO PERRONE (Persona)
• MICHELE SOLFRIZZO (Unità di personale interno)
• GIUSEPPINA MULE' (Persona)
• ANTONIA GALLO (Persona)

Prodotto

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Editore di

• American Society Of Microbiology (ASM) (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Applied and environmental microbiology (Rivista)

Insieme di parole chiave di

• Keywords of "New insight into the ochratoxin A biosynthetic pathway through deletion of a nonribosomal peptide synthetase gene in Aspergillus carbonarius." (Insieme di parole chiave)