http://www.cnr.it/ontology/cnr/individuo/prodotto/ID192305
Nonlinear protein - nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells (Articolo in rivista)
- Type
- Label
- Nonlinear protein - nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells (Articolo in rivista) (literal)
- Anno
- 2012-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1002/lapl.201110122 (literal)
- Alternative label
Altucci C., Nebbioso A., Benedetti R., Esposito R., Carafa V., Conte M., Micciarelli M., Altucci L., Velotta R. (2012)
Nonlinear protein - nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells
in Laser physics letters (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Altucci C., Nebbioso A., Benedetti R., Esposito R., Carafa V., Conte M., Micciarelli M., Altucci L., Velotta R. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Univ Naples Federico 2, CNISM, Naples, Italy
Univ Naples Federico 2, Dipartimento Sci Fis, Naples, Italy
Univ Naples 2, Dipartimento Patol Gen, Naples, Italy
IGB CNR, Naples, Italy (literal)
- Titolo
- Nonlinear protein - nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells (literal)
- Abstract
- We investigate the interactions between proteins and DNA in solutions of living cells, triggered by UV, 200-fs laser pulses. DNA-protein crosslinking is obtained with two different techniques: conventional chemical or laser-induced. Our results evidence a nonlinear response of the cells to the laser irradiation in the investigated intensity range. The laser induced crosslink efficiency can reach a value approximately twice higher than that obtained with ordinary chemical methods. The experimental results are very well reproduced by a simple phenomenological model based on the interplay between two-photon absorption by DNA bases and cell damage induced by high intensity laser pulse. (literal)
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