Nonlinear protein - nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells (Articolo in rivista)

Type
Label
  • Nonlinear protein - nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/lapl.201110122 (literal)
Alternative label
  • Altucci C., Nebbioso A., Benedetti R., Esposito R., Carafa V., Conte M., Micciarelli M., Altucci L., Velotta R. (2012)
    Nonlinear protein - nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells
    in Laser physics letters (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Altucci C., Nebbioso A., Benedetti R., Esposito R., Carafa V., Conte M., Micciarelli M., Altucci L., Velotta R. (literal)
Pagina inizio
  • 234 (literal)
Pagina fine
  • 239 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 9 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 3 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Univ Naples Federico 2, CNISM, Naples, Italy Univ Naples Federico 2, Dipartimento Sci Fis, Naples, Italy Univ Naples 2, Dipartimento Patol Gen, Naples, Italy IGB CNR, Naples, Italy (literal)
Titolo
  • Nonlinear protein - nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells (literal)
Abstract
  • We investigate the interactions between proteins and DNA in solutions of living cells, triggered by UV, 200-fs laser pulses. DNA-protein crosslinking is obtained with two different techniques: conventional chemical or laser-induced. Our results evidence a nonlinear response of the cells to the laser irradiation in the investigated intensity range. The laser induced crosslink efficiency can reach a value approximately twice higher than that obtained with ordinary chemical methods. The experimental results are very well reproduced by a simple phenomenological model based on the interplay between two-photon absorption by DNA bases and cell damage induced by high intensity laser pulse. (literal)
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