http://www.cnr.it/ontology/cnr/individuo/prodotto/ID191953

Isolation of Squarebop I bacteriorhodopsin from biomass of coastal salterns (Articolo in rivista)

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Isolation of Squarebop I bacteriorhodopsin from biomass of coastal salterns (Articolo in rivista) (literal)

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Lobasso S.(a), Lopalco P.(b), Angelini R.(a), Pollice A.(c), Laera G.(c), Milano F.(d), Agostiano A.(d,e), Corcelli A. (a,d) (2012)
Isolation of Squarebop I bacteriorhodopsin from biomass of coastal salterns
in Protein expression and purification (Print)
(literal)

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Lobasso S.(a), Lopalco P.(b), Angelini R.(a), Pollice A.(c), Laera G.(c), Milano F.(d), Agostiano A.(d,e), Corcelli A. (a,d) (literal)

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a Department of Basic Medical Sciences, University of Bari ''Aldo Moro'', Bari, Italy b Institute of Microelectronics and Microsystems-National Research Council, IMM-CNR, Lecce, Italy c Water Research Institute-National Research Council, IRSA-CNR, Bari, Italy d Institute for Chemical-Physical Processes-National Research Council, IPCF-CNR, Bari, Italy e Department of Chemistry, University of Bari ''Aldo Moro'', Bari, Italy (literal)

Titolo

Isolation of Squarebop I bacteriorhodopsin from biomass of coastal salterns (literal)

Abstract

Squarebop I bacteriorhodopsin is a light-activated proton pump present in the membranes of the archeon Haloquadratum walsbyi, a square-shaped organism representing 50-60% of microbial population in the crystallizer ponds of the coastal salterns. Here we describe: (1) the operating mode of a bioreactor designed to concentrate the saltern biomass through a microfiltration process based on polyethersulfone hollow fibers; (2) the isolation of Squarebop I bacteriorhodopsin from solubilized biomass by means of a single chromatographic step; (3) tightly bound lipids to the isolated and purified protein as revealed by MALDI-TOF/MS analysis; (4) the photoactivity of Squarebop I bacteriorhodopsin isolated from environmental samples by flash spectroscopy. Yield of the isolation process is 150 lg of Squarebop I bacteriorhodopsin from 1 l of 25-fold concentrated biomass. The possibility of using the concentrated biomass of salterns, as renewable resource for the isolation of functional bacteriorhodopsin and possibly other valuable bioproducts, is briefly discussed. (literal)

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