Expression and characterization of two new alkane inducible cytochrome P450s from Trichoderma harzianum (Articolo in rivista)

Type
Label
  • Expression and characterization of two new alkane inducible cytochrome P450s from Trichoderma harzianum (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s10529-011-0557-0 (literal)
Alternative label
  • R. Del Carratore, P. G. Gervasi , M. P. Contini, P. Beffy, B. E. Maserti, G. Giovannetti , A. Brondolo, V. Longo (2011)
    Expression and characterization of two new alkane inducible cytochrome P450s from Trichoderma harzianum
    in Biotechnology letters
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • R. Del Carratore, P. G. Gervasi , M. P. Contini, P. Beffy, B. E. Maserti, G. Giovannetti , A. Brondolo, V. Longo (literal)
Pagina inizio
  • 1201 (literal)
Pagina fine
  • 1206 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://link.springer.com/article/10.1007%2Fs10529-011-0557-0 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 33 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Clinical Physiology, National Research Council (CNR), via Moruzzi 1, Pisa, Italy Institute of Agricultural Biology and Biotechnology, CNR, via Moruzzi 1, Pisa, Italy Institute of Biophysics, CNR, via Moruzzi 1, Pisa, Italy Experimental Culture Centre, Valle D'Aosta, Fraz. Olleyes 9, Quart, AO, Italy (literal)
Titolo
  • Expression and characterization of two new alkane inducible cytochrome P450s from Trichoderma harzianum (literal)
Abstract
  • n-Dodecane and fatty acids were good inducers of cytochrome P450 (CYP) and the x-hydroxylase of lauric acid, which is a marker for x-hydroxylation of n-alkanes, in Trichoderma harzianum. A cDNA, containing an ORF of 1520 bp, encoding a CYP52 of 520 amino acids, was isolated by RACE. Another n-alkane-inducible CYP was identified by LLC-MS/MS analysis of a microsomal protein band induced by n-dodecane in a library of T. harzianum. This suggests that T. harzianum has a CYP-dependent conversion of alkanes to fatty acids allowing their incorporation into lipids. (literal)
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