http://www.cnr.it/ontology/cnr/individuo/prodotto/ID190873

Effects of resveratrol on HepG2 cells as revealed by 1H-NMR based metabolic profiling (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Effects of resveratrol on HepG2 cells as revealed by 1H-NMR based metabolic profiling (Articolo in rivista) (literal)

Anno

2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.bbagen.2011.10.005 (literal)

Alternative label

M. Massimi, A. Tomassini, F. Sciubba, A. P. Sobolev, L. Conti Devirgiliis, A. Miccheli (2012)
Effects of resveratrol on HepG2 cells as revealed by 1H-NMR based metabolic profiling
in Biochimica et biophysica acta. G, General subjects (Print); Elsevier BV, Amsterdam (Paesi Bassi)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

M. Massimi, A. Tomassini, F. Sciubba, A. P. Sobolev, L. Conti Devirgiliis, A. Miccheli (literal)

Pagina inizio

1 (literal)

Pagina fine

8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

1820 (literal)

Rivista

Biochimica et biophysica acta. G, General subjects (Rivista)

Note

Scopus (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Department of Basic and Applied Biology, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy Istituto di Metodologie Chimiche, Laboratorio di Risonanza Magnetica \"Annalaura Segre\" - CNR, 00015, Monterotondo, Rome, Italy Department of Biology and Biotechnologies \"Charles Darwin\", Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy (literal)

Titolo

Effects of resveratrol on HepG2 cells as revealed by 1H-NMR based metabolic profiling (literal)

Abstract

Background: Resveratrol, a polyphenol found in plant products, has been shown to regulate many cellular processes and to display multiple protective and therapeutic effects. Several in vitro and in vivo studies have demonstrated the influence of resveratrol on multiple intracellular targets that may regulate metabolic homeostasis. Methods: We analysed the metabolic modifications induced by resveratrol treatment in a human hepatoblastoma line, HepG2 cells, using a 1H-NMR spectroscopy-based metabolomics approach that allows the simultaneous screening of multiple metabolic pathways. Results: Results demonstrated that cells cultured in the presence or absence of resveratrol displayed different metabolic profiles: the treatment induced a decreased utilisation of glucose and amino acids for purposes of energy production and synthesis associated to a decreased release of lactate in the culture medium and an increase in succinate utilisation. At the same time, resveratrol treatment slowed the cell cycle in the S phase without inducing apoptosis, and increased Sirt1 expression, also affecting its intracellular localisation. Conclusions: Our results show that the metabolomic analysis of the exometabolome of resveratrol-treated HepG2 cells indicates a metabolic switch from glucose and amino acid utilisation to fat utilisation for the production of energy, and seem in agreement with an effect mediated via AMPK- and Sirt1-activation. General significance: NMR-based metabolomics has been applied in a hepatocyte cell culture model in relation to resveratrol treatment; such an approach could be transferred to evaluate the effects of nutritional compounds with health impact. (literal)

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