Expression of the serotonin transporter (SERT) in the choroid plexuses from buffalo brain (Articolo in rivista)

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  • Expression of the serotonin transporter (SERT) in the choroid plexuses from buffalo brain (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/ar.20610 (literal)
Alternative label
  • Pavone LM, Tafuri S, Mastellone V, Morte RD, Lombardi P, Avallone L, Maharajan V, Staiano N, Scala G. (2007)
    Expression of the serotonin transporter (SERT) in the choroid plexuses from buffalo brain
    in The anatomical record (Hoboken, N.J. : 2007) (Hoboken, N.J. : 2007)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Pavone LM, Tafuri S, Mastellone V, Morte RD, Lombardi P, Avallone L, Maharajan V, Staiano N, Scala G. (literal)
Pagina inizio
  • 1492 (literal)
Pagina fine
  • 1499 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 290 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Naples, Italy ; Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II, Naples, Italy ; Department of Experimental and Clinical Medicine ''G. Salvatore'', University of Magna Graecia, Viale Europa, Germaneto, Catanzaro, Italy ; CNR, Institute of Cybernetics, Pozzuoli (NA), Italy (literal)
Titolo
  • Expression of the serotonin transporter (SERT) in the choroid plexuses from buffalo brain (literal)
Abstract
  • Choroid plexuses (CPs) play pivotal roles in a wide range of processes that establish, survey, and maintain the biochemical and cellular status of the central nervous system. Mammalian CPs contain a very high density of serotonin receptors, and serotonin has been shown to affect CP functions. The serotonin transporter (SERT) regulates the entire serotonergic system, including serotonin receptors by means of modulation of serotonin concen-tration in the extracellular fluid. In this study, the expression of SERT in the CPs from the brain of a mammalian species,Bubalis bubalis, was estab-lished. By immunogold labeling in scanning electron microscopy, SERT immunoreactivity was found to be localized on the apical surface of the choroid epithelium. In particular, SERT positivity was detected on the apical portion of villi, and both on the membrane and in the cytoplasm of grouped cells on the surface of the choroid epithelium. Significantly, no SERT was detected in blood vessels irrigating the CPs. The expression of SERT mRNA transcripts of 440 bp in the CPs was detected by reverse-tran-scription polymerase chain reaction, and Western blotting analysis revealed the presence of three isoforms of the protein with molecular masses of approximately 70, 80, and 140 kDa, respectively, probably corresponding to differently glycosylated SERT. Our findings provide the first report of SERT detection in the CPs of buffalo brain and indicate that this protein is locally synthesized from the choroid epithelial cells. We suggest that SERT might have an important role in mammalian CPs, possibly regulating the serotonin flow between brain and rest of the body. (literal)
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