http://www.cnr.it/ontology/cnr/individuo/prodotto/ID190539

Less than 5 Netrin-1 molecules initiate attraction but 200 Sema3A molecules are necessary for repulsion (Articolo in rivista)

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Label

Less than 5 Netrin-1 molecules initiate attraction but 200 Sema3A molecules are necessary for repulsion (Articolo in rivista) (literal)

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Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Pinato G, Cojoc D, Lien LT, Ansuini A, Ban J, D'Este E, Torre V (2012)
Less than 5 Netrin-1 molecules initiate attraction but 200 Sema3A molecules are necessary for repulsion
in Scientific reports (Nature Publishing Group); Nature Publishing Group, New York (Stati Uniti d'America)
(literal)

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CNR-IOM Trieste (Pinato, Cojoc, D'Este) SISSA Trieste (Lien, Ansuini, Ban, Torre) (literal)

Titolo

Less than 5 Netrin-1 molecules initiate attraction but 200 Sema3A molecules are necessary for repulsion (literal)

Abstract

Guidance molecules, such as Sema3A or Netrin-1, induce growth cone (GC) repulsion or attraction. In order to determine the speed of action and efficiency of these guidance cues we developed an experimental procedure to deliver controlled amounts of these molecules. Lipid vesicles encapsulating 10-104 molecules of Sema3A or Netrin-1 were manipulated with high spatial and temporal resolution by optical tweezers and their photolysis triggered by laser pulses. Guidance molecules released from the vesicles diffused and reached the GC membrane in a few seconds. Following their arrival, GCs retracted or grew in 20-120 s. By determining the number of guidance molecules trapped inside vesicles and estimating the fraction of guidance molecules reaching the GC, we show that the arrival of less than 5 Netrin-1 molecules on the GC membrane is sufficient to induce growth. In contrast, the arrival of about 200 Sema3A molecules is necessary to induce filopodia repulsion. (literal)

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