http://www.cnr.it/ontology/cnr/individuo/prodotto/ID190237
Live-cell-permeant thiophene fluorophores and cell-mediated formation of fluorescent fibrils (Articolo in rivista)
- Type
- Label
- Live-cell-permeant thiophene fluorophores and cell-mediated formation of fluorescent fibrils (Articolo in rivista) (literal)
- Anno
- 2011-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1021/ja2065522 (literal)
- Alternative label
I. E. Palamà, F. Di Maria, I. Viola, E. Fabiano, G. Gigli, C. Bettini, G. Barbarella (2011)
Live-cell-permeant thiophene fluorophores and cell-mediated formation of fluorescent fibrils
in Journal of the American Chemical Society (Online); ACS, American chemical society, Washington, DC (Stati Uniti d'America)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- I. E. Palamà, F. Di Maria, I. Viola, E. Fabiano, G. Gigli, C. Bettini, G. Barbarella (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
- http://dx.doi.org/10.1021/ja2065522 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- NNL-CNR Nanoscience Institute and Dip. Ingegneria Innovazione, Università del Salento, Via Arnesano, 73100 Lecce, Italy
Istitute for Organic Synthesis and Photoreactivity and Mediteknology srl, National Research Council, Via Gobetti 101, 40129 Bologna, Italy
NNL, Istituto Nanoscienze - CNR, via Arnesano, 73100 Lecce, and c/o Dipartimento di Fisica, Università La Sapienza, Rome, Italy (literal)
- Titolo
- Live-cell-permeant thiophene fluorophores and cell-mediated formation of fluorescent fibrils (literal)
- Abstract
- In our search for thiophene fluorophores that can overcome the limits of currently available organic dyes in live-cell staining, we synthesized biocompatible dithienothiophene-S,S-dioxide derivatives (DTTOs) that were spontaneously taken up by live mouse embryonic fibroblasts and HeLa cells. Upon treatment with DTTOs, the cells secreted nanostructured fluorescent fibrils, while cell viability remained unaltered. Comparison with the behavior of other cell-permeant, newly synthesized thiophene fluorophores showed that the formation of fluorescent fibrils was peculiar to DTTO dyes. Laser scanning confocal microscopy of the fluorescent fibrils showed that most of them were characterized by helical supramolecular organization. Electrophoretic analysis and theoretical calculations suggested that the DTTOs were selectively recognized by the HyPro component of procollagen polypeptide chains and incorporated through the formation of multiple H-bondings. (literal)
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- Autore CNR
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