Transcript profiling of the common bean - Rhizobium symbiosis under oxidative stress (Abstract/Poster in atti di convegno)

Type
Label
  • Transcript profiling of the common bean - Rhizobium symbiosis under oxidative stress (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Alternative label
  • Ramirez M, Guillén G, Fuentes SI, Iñiguez LP, Aparicio-Fabre R, Rivera P, Salazar E, Encarnación-Guevara S, Panzeri D, Castiglioni B , Cremonesi P, Strozzi F, Stella A, Girard L, Sparvoli F, Hernandez G (2012)
    Transcript profiling of the common bean - Rhizobium symbiosis under oxidative stress
    in VI International Conference on Legume Genetics and Genomics (ICLGG), October 2-7, 2012, Hyderabad, India, Hyderabad, India, October 2-7, 2012,
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Ramirez M, Guillén G, Fuentes SI, Iñiguez LP, Aparicio-Fabre R, Rivera P, Salazar E, Encarnación-Guevara S, Panzeri D, Castiglioni B , Cremonesi P, Strozzi F, Stella A, Girard L, Sparvoli F, Hernandez G (literal)
Note
  • Abstract (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • RM, FSI, ILP, AFR, RP, SE, EGS, HG, GL - Centro de Ciencias Cenómicas - Universidad Nacional Autónoma de México, Cuernavaca, México GG - Instituto de Biotecnología - Universidad Nacional Autónoma de México, Cuernavaca, México PD, CB, CP, SA, SF - Istituto di Biologia e Biotecnologia Agraria, Milano, Italy (literal)
Titolo
  • Transcript profiling of the common bean - Rhizobium symbiosis under oxidative stress (literal)
Abstract
  • A wide range of environmental stresses lead to an elevated production of reactive oxygen species (ROS) in plant cells thus resulting in oxidative stress. The biological nitrogen fixation in the legume - Rhizobium symbiosis is at high risk of damage from oxidative stress. Common bean (Phaseolus vulgaris) active nodules exposed to the herbicide Paraquat (1,1 '-Dimethyl-4, 4'-bipyridinium dichloride hydrate) that generates ROS accumulation, showed a reduced nitrogenase activity and ureide content. We analyzed the global gene response of stressed nodules using the Bean CombiMatrix Custom Array 90K, that includes probes from some 30,000 expressed sequence tags (EST). A total of 4,280 ESTs were differentially expressed in oxidative stressed bean nodules; of these 2,218 were repressed. These genes were grouped in 44 different biological processes as defined by Gene Onthology. Analysis with the PathExpress bioinformatic tool, adapted for bean, identified five significantly repressed metabolic pathways related to carbon/nitrogen metabolism that is crucial for nodule function. The expression platform for transcription factor (TF) genes based in qRT-PCR revealed that 67 TF genes were differentially expressed in oxidative stressed nodules. The expression analysis of fifteen oxidative stress responsive genes from the bacteroids in stressed nodules revealed that only few genes showed significant changes in their expression level. (literal)
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