Identification of Clostridium beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum isolated from silage, raw milk and hard cheese by a multiplex PCR assay. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Identification of Clostridium beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum isolated from silage, raw milk and hard cheese by a multiplex PCR assay. (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1017/S002202991200026X (literal)

Alternative label

• Paola Cremonesi; Laura Vanoni; Tiziana Silvetti; Stefano Morandi; Milena Brasca (2012)
  Identification of Clostridium beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum isolated from silage, raw milk and hard cheese by a multiplex PCR assay.
  in Journal of dairy research (Online)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Paola Cremonesi; Laura Vanoni; Tiziana Silvetti; Stefano Morandi; Milena Brasca (literal)

Pagina inizio

• 318 (literal)

Pagina fine

• 323 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 79 (literal)

Rivista

• Journal of dairy research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 6 (literal)

Note

• ISI Web of Science (WOS) (literal)
• Scopus (literal)
• PubMed (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• PC, IBBA-CNR LV, TS, SM, MB, ISPA-CNR (literal)

Titolo

• Identification of Clostridium beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum isolated from silage, raw milk and hard cheese by a multiplex PCR assay. (literal)

Abstract

• Late blowing, caused by the outgrowth of clostridial spores present in raw milk and originating from silage, can create considerable product loss, especially in the production of hard and semi-hard cheeses. The conventional method for the isolation of Clostridium spp. from cheeses with lateblowing symptoms is very complicated and the identification of isolates is problematic. The aim of this work was the development of a multiplex PCR method for the detection of the main dairy-related clostridia such as: Cl. beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum. Samples derived from silage, raw milk and hard cheese were analysed by the most probable number (MPN) enumeration. Forty-four bacterial strains isolated from gas positive tubes were used to check the reliability of the multiplex PCR assay. The specificity of the primers was tested by individually analysing each primer pair and the primer pair combined in the multiplex PCR. It was interesting to note that the samples not identified by the multiplex PCR assay were amplified by V2-V3 16S rRNA primer pair and the sequencing revealed the aligned 16S rRNA sequences to be Paenibacillus and Bacillus spp. This new molecular assay provides a simple promising alternative to traditional microbiological methods for a rapid, sensitive detection of clostridia in dairy products. (literal)

Prodotto di

• Conservazione delle risorse genetiche animali e valorizzazione delle loro produzioni (AG.P01.020.001) (Modulo)
• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR

• PAOLA CREMONESI (Unità di personale interno)
• STEFANO MORANDI (Persona)
• MILENA BRASCA (Unità di personale interno)
• LAURA VANONI (Unità di personale esterno)
• TIZIANA SILVETTI (Persona)

Insieme di parole chiave

• Keywords of "Identification of Clostridium beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum isolated from silage, raw milk and hard cheese by a multiplex PCR assay." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Conservazione delle risorse genetiche animali e valorizzazione delle loro produzioni (AG.P01.020.001) (Modulo)

Autore CNR di

• MILENA BRASCA (Unità di personale interno)
• TIZIANA SILVETTI (Persona)
• LAURA VANONI (Unità di personale esterno)
• STEFANO MORANDI (Persona)
• PAOLA CREMONESI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of dairy research (Rivista)

Insieme di parole chiave di

• Keywords of "Identification of Clostridium beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum isolated from silage, raw milk and hard cheese by a multiplex PCR assay." (Insieme di parole chiave)