Severity of left ventricular dysfunction in heart failure patients affects the degree of serum-induced cardiomyocyte apoptosis. Importance of inflammatory response and metabolism (Articolo in rivista)

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  • Severity of left ventricular dysfunction in heart failure patients affects the degree of serum-induced cardiomyocyte apoptosis. Importance of inflammatory response and metabolism (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.ijcard.2012.07.025 (literal)
Alternative label
  • Claudia Consoli, Lucia Gatta, Ferdinando Iellamo, Francesca Molinari, Giuseppe M.C. Rosano, Lionel N.J.L. Marlier (2013)
    Severity of left ventricular dysfunction in heart failure patients affects the degree of serum-induced cardiomyocyte apoptosis. Importance of inflammatory response and metabolism
    in International journal of cardiology (print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Claudia Consoli, Lucia Gatta, Ferdinando Iellamo, Francesca Molinari, Giuseppe M.C. Rosano, Lionel N.J.L. Marlier (literal)
Pagina inizio
  • 2859 (literal)
Pagina fine
  • 2866 (literal)
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  • http://www.internationaljournalofcardiology.com/article/S0167-5273(12)00977-1/abstract (literal)
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  • 167 (literal)
Rivista
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  • 8 (literal)
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  • 6 (literal)
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  • Centre for Clinical & Basic Research, Cardiovascular Research Unit, Department. of Medical Sciences, IRCCS San Raffaele Pisana, Rome Institute of Translational Pharmacology (IFT), National Research Council (CNR) Rome (literal)
Titolo
  • Severity of left ventricular dysfunction in heart failure patients affects the degree of serum-induced cardiomyocyte apoptosis. Importance of inflammatory response and metabolism (literal)
Abstract
  • Background/Objectives In heart failure pro-inflammatory cytokines contribute to cardiomyocytes loss by apoptosis and play a role in the remodelling of the extracellular matrix (ECM). Myocardial injury recruits endothelial progenitor cells (EPCs) to the site of damage and stimulates their differentiation, contributing to myocardial tissue repair. We investigated if the severity of left ventricular dysfunction in heart failure patients (HF) may influence the ability of serum to induce cardiomyocytes death and whether this effect is affected by inflammation and intracellular oxidative stress pathways. Methods Adult murine cardiomyocytes HL-5 were incubated with 2% human serum from patients with heart failure (NYHA classes I to IV). Apoptosis was analysed by two different methods. TNF-?, IL-1?, IL-6, matrix metalloproteinase 1 (MMP-1) and tissue inhibitor of metalloproteinases 1 (TIMP-1) were measured in sera from patients. Results Cytokine levels were higher in sera from moderate-severe CHF compared to that of patients with mild CHF. Levels of CD117+ (c-Kit+) cells and EPCs were significantly lower in blood from moderate-severe HF patients. Serum from HF patients induced a significantly higher ROS production involving p38 MAPK signalling and apoptosis in cardiomyocytes. NAC treatment prevented serum-induced oxidative effects. The increase of AMPK phosphorylation showed an involvement of FFA ?-oxidation during apoptotic stress. Conclusions All these alterations could be used as predictive factors of worsening in heart failure and culture of cardiomyocytes could be employed to test pharmacological effects. (literal)
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