Glutathione Is Required by Rhizobium etli for Glutamine Utilization and Symbiotic Effectiveness (Articolo in rivista)

Type
Label
  • Glutathione Is Required by Rhizobium etli for Glutamine Utilization and Symbiotic Effectiveness (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1094/MPMI-06-11-0163 (literal)
Alternative label
  • Tatè, R.; Cermola M.; Riccio A.; Diez-Roux G.; Patriarca E.J. (2012)
    Glutathione Is Required by Rhizobium etli for Glutamine Utilization and Symbiotic Effectiveness
    in Molecular plant-microbe interactions
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Tatè, R.; Cermola M.; Riccio A.; Diez-Roux G.; Patriarca E.J. (literal)
Pagina inizio
  • 331 (literal)
Pagina fine
  • 340 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 25 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 3 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • A Buzzati Traverso CNR, Inst Genet & Biophys, I-80131 Naples, Italy TIGEM, I-80131 Naples, Italy (literal)
Titolo
  • Glutathione Is Required by Rhizobium etli for Glutamine Utilization and Symbiotic Effectiveness (literal)
Abstract
  • Here, we provide genetic and biochemical evidence indicating that the ability of Rhizobium etli bacteria to efficiently catabolize glutamine depends on its ability to produce reduced glutathione (L-gamma-glutamyl-L-cysteinylglycine [[GSH]]). We find that GSH-deficient strains, namely a gshB (GSH synthetase) and a gor (GSH reductase) mutant, can use different amino acids, including histidine, alanine, and asparagine but not glutamine, as sole source of carbon, energy, and nitrogen. Moreover, L-buthionine(S,R)-sulfoximine, a GSH synthesis inhibitor, or diamide that oxidizes GSH, induced the same phenotype in the wild-type strain. Among the steps required for its utilization, glutamine uptake, occurring through the two well-characterized carriers (Aap and Bra systems) but not glutamine degradation or respiration, was largely reduced in GSH-deficient strains. Furthermore, GSH-deficient mutants of R. etli showed a reduced symbiotic efficiency. Exogenous GSH was sufficient to rescue glutamine uptake or degradation ability, as well as the symbiotic effectiveness of GSH mutants. Our results suggest a previously unknown GSH-glutamine metabolic relationship in bacteria. (literal)
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