http://www.cnr.it/ontology/cnr/individuo/prodotto/ID189425
RhoB regulates uPAR signalling (Articolo in rivista)
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- Label
- RhoB regulates uPAR signalling (Articolo in rivista) (literal)
- Anno
- 2012-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1242/jcs.091579 (literal)
- Alternative label
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- Alfano D.; Ragno P.; Stoppelli M.P.; Ridley A. J. (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Kings Coll London, Randall Div Cell & Mol Biophys, London SE1 1UL, England
Univ Salerno, Dept Biol & Chem, I-84084 Salerno, Italy
CNR, Inst Genet & Biophys Adriano Buzzati Traverso, I-80131 Naples, Italy (literal)
- Titolo
- RhoB regulates uPAR signalling (literal)
- Abstract
- Urokinase-type plasminogen activator (uPA) and its receptor, uPAR, play important roles in promoting cancer cell adhesion, migration and invasion. Rho GTPases are key coordinators of these processes; the Rho GTPase Rac1 has previously been implicated in uPA-and/or uPAR-induced migratory or morphological cell responses. We used RNAi to deplete 12 different Rho GTPases to screen for effects on uPA-stimulated migration, and found that depletion of RhoB significantly reduces uPA-induced migration and invasion of prostate carcinoma cells. RhoB depletion did not affect the expression or surface levels of uPAR but reduced the uPAR-induced increase in levels of several integrins and inhibited uPAR signalling to the actin regulator cofilin, the cell-adhesion signal-transduction adaptor molecule paxillin and the serine/threonine kinase Akt. uPAR rapidly activated RhoB and increased RhoB expression. RhoB depletion also reduced cell adhesion to and spreading on vitronectin, which is a uPAR ligand. This correlated with decreased association between integrins and uPAR and reduced integrin beta 1 activity. Our results indicate that RhoB is a key regulator of uPAR signalling in cell adhesion, migration and invasion. (literal)
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