Cardiomyogenic Commitment of Human Placental derived Stem Cells grown on Combined Synthetic-Fibrin Scaffold through (Abstract/Poster in convegno)

Type

• Prodotto della ricerca (Classe)
• Abstract/Poster in convegno (Classe)

Label

• Cardiomyogenic Commitment of Human Placental derived Stem Cells grown on Combined Synthetic-Fibrin Scaffold through (Abstract/Poster in convegno) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Alternative label

• Mario Ledda, Enrica Briganti, Paola Losi, Cristiana Mattei, Settimio Grimaldi, Rodolfo Marchese, Giorgio Soldani, Antonella Lisi (2012)
  Cardiomyogenic Commitment of Human Placental derived Stem Cells grown on Combined Synthetic-Fibrin Scaffold through
  in 3rd Lugano Stem Cell Meeting, Lugano, Switzerland, 25-26/06/12
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Mario Ledda, Enrica Briganti, Paola Losi, Cristiana Mattei, Settimio Grimaldi, Rodolfo Marchese, Giorgio Soldani, Antonella Lisi (literal)

Note

• Poster (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute of Translational Pharmacology, C.N.R., Roma, 00133, Italy Institute of Clinical Physiology, C.N.R., Massa, 54100, Italy Research Center, FBF S. Peter Hospital, Rome, Italy (literal)

Titolo

• Cardiomyogenic Commitment of Human Placental derived Stem Cells grown on Combined Synthetic-Fibrin Scaffold through (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autoriVolume

• Stem cell therapy and tissue engineering are actually considered very promising approaches for the treatment of damaged myocardium. A suitable cell delivery system approach requires an appropriate stem cell source able to proliferate and differentiate, but also to be easily handled and have elastic properties without affecting the cardiac contractile function. Mesenchymal Stem Cells (MSCs) isolated from term Amniotic membrane (AM) are emerging as a very attractive source for cardiac repair, due to their multi-lineage differentiation potential and low immunogenic properties. In this study an elastic scaffold, obtained combining a fibrin layer with a poly(ether)urethane-polydimethylsiloxane (PEtU-PDMS) layer, was used as a substrate for in vitro studies of human amniotic mesenchymal stromal cell (hAMSCs) growth and differentiation. Cell proliferation and metabolic activity of hAMSCs seeded on the fibrin scaffold were evaluated by bromodeoxyuridine and WST-1 assays, for up to 14 days of culture. The beginning of cardiomyogenic commitment of hAMSCs grown on the scaffold was induced, for the first time in this cell population, by a nitric oxide (NO) treatment. The NO treated hAMSCs, showed morphological changes using immunofluorescence analysis, an increase of the messenger cardiac differentiation markers [troponin I (TnI) and NK2 transcription factor related locus 5 (Nkx2.5)] and a modulation of the endothelial markers [vascular endothelial growth factor (VEGF) and kinase insert domain receptor (KDR)] by RT-PCR analysis. The results of this study suggest that the PEtU-PDMS/fibrin combined scaffold allows a better proliferation and metabolic activity of hAMSCs and also the beginning of the differentiation process towards a cardiomyogenic lineage representing a new cell delivery system that could be used in cardiac regenerative medicine. (literal)

Prodotto di

• La Risonanza di ciclotrone come strategia per la veicolazione e il rilascio di farmaci (ME.P06.031.001) (Modulo)
• Sviluppo di protocolli per il differenziamento di cellule staminali tramite interazione tra frequenze di risonanza di ciclotrone di ioni e biosistemi (ME.P06.031.003) (Modulo)

Autore CNR

• SETTIMIO GRIMALDI (Persona)
• MARIO LEDDA (Persona)
• PAOLA LOSI (Unità di personale interno)
• ANTONELLA LISI (Unità di personale interno)
• ENRICA BRIGANTI (Unità di personale interno)
• GIORGIO SOLDANI (Unità di personale interno)

Incoming links:

Autore CNR di

• ANTONELLA LISI (Unità di personale interno)
• MARIO LEDDA (Persona)
• SETTIMIO GRIMALDI (Persona)
• GIORGIO SOLDANI (Unità di personale interno)
• ENRICA BRIGANTI (Unità di personale interno)
• PAOLA LOSI (Unità di personale interno)

Prodotto

• La Risonanza di ciclotrone come strategia per la veicolazione e il rilascio di farmaci (ME.P06.031.001) (Modulo)
• Sviluppo di protocolli per il differenziamento di cellule staminali tramite interazione tra frequenze di risonanza di ciclotrone di ioni e biosistemi (ME.P06.031.003) (Modulo)