A combined synthetic-fibrin scaffold supports growth and cardiomyogenic commitment of human placental derived stem cells. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• A combined synthetic-fibrin scaffold supports growth and cardiomyogenic commitment of human placental derived stem cells. (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Alternative label

• Antonella Lisi,1 Enrica Briganti,2 Mario Ledda,1 Paola Losi,2 Settimio Grimaldi,1 Rodolfo Marchese,3 and Giorgio Soldani2 (2012)
  A combined synthetic-fibrin scaffold supports growth and cardiomyogenic commitment of human placental derived stem cells.
  in PloS one
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Antonella Lisi,1 Enrica Briganti,2 Mario Ledda,1 Paola Losi,2 Settimio Grimaldi,1 Rodolfo Marchese,3 and Giorgio Soldani2 (literal)

Pagina inizio

• e34284 1 (literal)

Pagina fine

• e34284 10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 7 (literal)

Rivista

• PloS one (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 4 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 Institute of Translational Pharmacology, National Research Council (C.N.R.), Roma, Italy 2 Institute of Clinical Physiology, National Research Council (C.N.R.), Massa, Italy 3 Research Center, S. Peter Hospital FBF, Rome, Italy (literal)

Titolo

• A combined synthetic-fibrin scaffold supports growth and cardiomyogenic commitment of human placental derived stem cells. (literal)

Abstract

• AIMS: A potential therapy for myocardial infarction is to deliver isolated stem cells to the infarcted site. A key issue with this therapy is to have at one's disposal a suitable cell delivery system which, besides being able to support cell proliferation and differentiation, may also provide handling and elastic properties which do not affect cardiac contractile function. In this study an elastic scaffold, obtained combining a poly(ether)urethane-polydimethylsiloxane (PEtU-PDMS) semi-interpenetrating polymeric network (s-IPN) with fibrin, was used as a substrate for in vitro studies of human amniotic mesenchymal stromal cells (hAMSC) growth and differentiation. METHODOLOGY/PRINCIPAL FINDINGS: After hAMSC seeding on the fibrin side of the scaffold, cell metabolic activity and proliferation were evaluated by WST-1 and bromodeoxyuridine assays. Morphological changes and mRNAs expression for cardiac differentiation markers in the hAMSCs were examined using immunofluorescence and RT-PCR analysis. The beginning of cardiomyogenic commitment of hAMSCs grown on the scaffold was induced, for the first time in this cell population, by a nitric oxide (NO) treatment. Following NO treatment hAMSCs show morphological changes, an increase of the messenger cardiac differentiation markers [troponin I (TnI) and NK2 transcription factor related locus 5 (Nkx2.5)] and a modulation of the endothelial markers [vascular endothelial growth factor (VEGF) and kinase insert domain receptor (KDR)]. CONCLUSIONS/SIGNIFICANCE: The results of this study suggest that the s-IPN PEtU-PDMS/fibrin combined scaffold allows a better proliferation and metabolic activity of hAMSCs cultured up to 14 days, compared to the ones grown on plastic dishes. In addition, the combined scaffold sustains the beginning of hAMSCs differentiation process towards a cardiomyogenic lineage. (literal)

Prodotto di

• Sviluppo di protocolli per il differenziamento di cellule staminali tramite interazione tra frequenze di risonanza di ciclotrone di ioni e biosistemi (ME.P06.031.003) (Modulo)
• La Risonanza di ciclotrone come strategia per la veicolazione e il rilascio di farmaci (ME.P06.031.001) (Modulo)

Autore CNR

• MARIO LEDDA (Persona)
• PAOLA LOSI (Unità di personale interno)
• GIORGIO SOLDANI (Unità di personale interno)
• ANTONELLA LISI (Unità di personale interno)
• ENRICA BRIGANTI (Unità di personale interno)
• SETTIMIO GRIMALDI (Persona)

Incoming links:

Autore CNR di

• ANTONELLA LISI (Unità di personale interno)
• MARIO LEDDA (Persona)
• SETTIMIO GRIMALDI (Persona)
• GIORGIO SOLDANI (Unità di personale interno)
• ENRICA BRIGANTI (Unità di personale interno)
• PAOLA LOSI (Unità di personale interno)

Prodotto

• La Risonanza di ciclotrone come strategia per la veicolazione e il rilascio di farmaci (ME.P06.031.001) (Modulo)
• Sviluppo di protocolli per il differenziamento di cellule staminali tramite interazione tra frequenze di risonanza di ciclotrone di ioni e biosistemi (ME.P06.031.003) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• PloS one (Rivista)