Sea urchin early histone H2A modulator binding factor 1 is a positive transcription factor also for the early histone H3 gene (Articolo in rivista)

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  • Sea urchin early histone H2A modulator binding factor 1 is a positive transcription factor also for the early histone H3 gene (Articolo in rivista) (literal)
Anno
  • 1993-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1073/pnas.90.14.6854 (literal)
Alternative label
  • Franco Palla, Celestino Bonurat, Letizia Anello, Caterina Casanot, Mirella Ciaccio and Giovanni Spinelli (1993)
    Sea urchin early histone H2A modulator binding factor 1 is a positive transcription factor also for the early histone H3 gene
    in Proceedings of the National Academy of Sciences of the United States of America; National Academy of Sciences, Washington (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Franco Palla, Celestino Bonurat, Letizia Anello, Caterina Casanot, Mirella Ciaccio and Giovanni Spinelli (literal)
Pagina inizio
  • 6854 (literal)
Pagina fine
  • 6858 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 90 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 5 (literal)
Note
  • PubMe (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Biologia dello Sviluppo del Consiglio Nazionale delle Ricerche, via Archirafi 20, 90123, Palermo, Italy; Dipartimento di Biologia Cellulare e dello Sviluppo; Istituto di Biologia dello Sviluppo del Consiglio Nazionale delle Ricerche, via Archirafi 20, 90123, Palermo, Italy; tDipartimento di Biologia Cellulare e dello Sviluppo; Dipartimento di Biologia Cellulare e dello Sviluppo; Istituto di Biologia dello Sviluppo del Consiglio Nazionale delle Ricerche, via Archirafi 20, 90123, Palermo, Italy; Dipartimento di Biologia Cellulare e dello Sviluppo (literal)
Titolo
  • Sea urchin early histone H2A modulator binding factor 1 is a positive transcription factor also for the early histone H3 gene (literal)
Abstract
  • To shed some light on the mechanisms involved in the coordinate regulation of the early histone gene set during sea urchin development, we tested the hypothesis that the upstream sequence element USE1, previously identified in the early H2A modulator, could also participate in the transcription of the early histone H3 gene. We found by DNase I protection analysis and by competition in electrophoretic mobility- shift experiments that two sequence elements of the H3 promoter closely resembled the USE1-H2A sequence in their binding activity for nuclear factors from 64-cell stage embryos. These modulator binding factor 1 (MBF-1)-related factors seem to recognize the ACAGA motif that is conserved between the USEl-like sequences of both H2A and H3 promoters. In fact, excess oligonucleotide containing a mutated USE1-H2A element in which the ACAGA sequence was mutated to AGTCA failed to compete with the USE1 sites of both H2A and H3 genes for interaction with MBF-1. Finally, in vivo transcriptional analysis in both Xenopus and sea urchin showed that an excess of USE1-H2A element efficiently competed for the activity of the H3 promoter. From these results we condude that MBF-1 is a transcription factor conserved between sea urchin and frog and that MBF-1 or related transcription factors are involved in the coordinate expression of both H2A and H3 early histone genes. (literal)
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