ENZYMATIC ACTIVITY, PROTEIN EXPRESSION, AND GENE SEQUENCE OF CRUZIPAIN IN VIRULENT AND ATTENUATED TRYPANOSOMA CRUZI STRAINS (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• ENZYMATIC ACTIVITY, PROTEIN EXPRESSION, AND GENE SEQUENCE OF CRUZIPAIN IN VIRULENT AND ATTENUATED TRYPANOSOMA CRUZI STRAINS (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Alternative label

• DUSCHAK V.G.; CIACCIO M.; NASSER J.R.; BASOMBRIO M.A. (2001)
  ENZYMATIC ACTIVITY, PROTEIN EXPRESSION, AND GENE SEQUENCE OF CRUZIPAIN IN VIRULENT AND ATTENUATED TRYPANOSOMA CRUZI STRAINS
  in Journal of bacteriology (Online)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• DUSCHAK V.G.; CIACCIO M.; NASSER J.R.; BASOMBRIO M.A. (literal)

Pagina inizio

• 1016 (literal)

Pagina fine

• 1022 (literal)

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• 87 (literal)

Rivista

• Journal of bacteriology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 7 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 5 (literal)

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• Laboratorio de Bioquímica de Parásitos, Instituto de Investigaciones Biotecnológicas, Universidad de San Martín, INTI: Av General Paz entre Albarellos y Constituyentes (Edificio 24), Casilla de Correo 30 (1650) San Martin, Provincia de Buenos Aires, Argentina Istituto di Biomedicina e immunologia molecolare, Consiglio Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italia. Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Buenos Aires 177, 4.400 Salta, Argentina. (literal)

Titolo

• ENZYMATIC ACTIVITY, PROTEIN EXPRESSION, AND GENE SEQUENCE OF CRUZIPAIN IN VIRULENT AND ATTENUATED TRYPANOSOMA CRUZI STRAINS (literal)

Abstract

• Protein expression, characterized in Western blots and gelatinolytic activity, of cruzipain (Cr), the major Trypanosoma cruzi cysteine proteinase, was compared among 3 attenuated T. cruzi strains (TUL 0, TCC, and Y null) and their virulent counterparts (TUL 2, Tulahuen, and Y). All attenuated strains displayed a weaker gelatinolytic activity as compared with their virulent counterparts. The electrophoretic mobility and immunological reactivity revealed quantitative and qualitative differences, with the attenuated parasites showing bands of less density in all strains and lower mobility in 2 of them, as compared with the virulent strains. Sequence analysis of 1 Cr gene in the Tulahuen and TCC strains indicated 37/1404 base pair substitutions, corresponding to 20 amino acid changes in the attenuated strain. A similar comparative analysis of 1 Cr gene between Y and Y null strains showed 13/1404 base pair substitutions, corresponding to 8 amino acid changes in the attenuated strain. Although enough variability exists in the Cr gene to allow for less- or nonfunctional isoforms of the protein, further clones should be analyzed to establish whether attenuation is regularly associated with specific sequence changes of this enzyme. (literal)

Prodotto di

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)

Autore CNR

• MIRELLA CIACCIO (Unità di personale interno)

Incoming links:

Prodotto

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)

Autore CNR di

• MIRELLA CIACCIO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of bacteriology (Rivista)