Micro flow cytometer with 3D hydrodynamic focusing (Contributo in atti di convegno)

Type

• Contributo in atti di convegno (Classe)
• Prodotto della ricerca (Classe)

Label

• Micro flow cytometer with 3D hydrodynamic focusing (Contributo in atti di convegno) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1117/12.908735 (literal)

Alternative label

• Testa G; Bernini. R (2012)
  Micro flow cytometer with 3D hydrodynamic focusing
  in Conference on Frontiers in Biological Detection - From Nanosensors to Systems IV, San Francisco, CA (USA), JAN 21-22, 2012
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Testa G; Bernini. R (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume

• FRONTIERS IN BIOLOGICAL DETECTION: FROM NANOSENSORS TO SYSTEMS IV (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#volumeInCollana

• 8212 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 82120H (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR IREA (literal)

Titolo

• Micro flow cytometer with 3D hydrodynamic focusing (literal)

Abstract

• This paper reports a micro flow cytometer fabricated in Polymethylmethacrylate (PMMA) in which a 3d hydrodynamic flow focusing is employed in order to align the particles in a single line along the focused stream. The device has been fabricated by direct micro milling of two parts of PMMA that were finally bonded together. With a suitable choice of the fluidic channel geometry, a circular sample stream located in the center of the channel is obtained. Numerical simulations have been performed in order to investigate the flow characteristic of the structure and find the desiderated geometry. Three dimensional hydrofocusing of the sample fluid was analysed and demonstrated by cross sectional fluorescence imaging in good agreement with numerical simulations. Flow cytometry measurements have been performed by using 10 mu m particles. From the analysis of the fluorescence signals collected at each transit event we can confirm that the device was capable of creating a single-file particle stream. The results show that the device was capable of discriminating single microparticles with a good signal-to-noise ratio and a high throughput. (literal)

Prodotto di

• Istituto per il rilevamento elettromagnetico dell'ambiente (IREA) (Istituto)
• Sensori e tecniche di elaborazione per l'imaging elettromagnetico nelle applicazioni di sicurezza fisica (INT.P01.008.001) (Modulo)

Autore CNR

• GENNI TESTA (Unità di personale interno)
• ROMEO BERNINI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Micro flow cytometer with 3D hydrodynamic focusing" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per il rilevamento elettromagnetico dell'ambiente (IREA) (Istituto)
• Sensori e tecniche di elaborazione per l'imaging elettromagnetico nelle applicazioni di sicurezza fisica (INT.P01.008.001) (Modulo)

Autore CNR di

• ROMEO BERNINI (Unità di personale interno)
• GENNI TESTA (Unità di personale interno)

Insieme di parole chiave di

• Parole chiave di "Micro flow cytometer with 3D hydrodynamic focusing" (Insieme di parole chiave)