RP-HPLC-ESI-MS evidenced that salivary cystatin B is detectable in adult human whole saliva mostly as S-modified derivatives: S-Glutathionyl, S-cysteinyl and S-S 2-mer (Articolo in rivista)

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  • RP-HPLC-ESI-MS evidenced that salivary cystatin B is detectable in adult human whole saliva mostly as S-modified derivatives: S-Glutathionyl, S-cysteinyl and S-S 2-mer (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.jprot.2011.10.006 (literal)
Alternative label
  • Cabras, Tiziana; Manconi, Barbara; Iavarone, Federica; Fanali, Chiara; Nemolato, Sonia; Fiorita, Antonella; Scarano, Emanuele; Passali, Giulio Cesare; Manni, Armando; Cordaro, Massimo; Paludetti, Gaetano; Faa, Gavino; Messana, Irene; Castagnola, Massimo (2012)
    RP-HPLC-ESI-MS evidenced that salivary cystatin B is detectable in adult human whole saliva mostly as S-modified derivatives: S-Glutathionyl, S-cysteinyl and S-S 2-mer
    in Journal of proteomics (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Cabras, Tiziana; Manconi, Barbara; Iavarone, Federica; Fanali, Chiara; Nemolato, Sonia; Fiorita, Antonella; Scarano, Emanuele; Passali, Giulio Cesare; Manni, Armando; Cordaro, Massimo; Paludetti, Gaetano; Faa, Gavino; Messana, Irene; Castagnola, Massimo (literal)
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  • 75 (literal)
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  • 6 (literal)
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  • ISI Web of Science (WoS) (literal)
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  • Catholic University of the Sacred Heart; Catholic University of the Sacred Heart; University of Cagliari; University of Cagliari; Catholic University of the Sacred Heart; Catholic University of the Sacred Heart (literal)
Titolo
  • RP-HPLC-ESI-MS evidenced that salivary cystatin B is detectable in adult human whole saliva mostly as S-modified derivatives: S-Glutathionyl, S-cysteinyl and S-S 2-mer (literal)
Abstract
  • An HPLC-ESI-MS analysis of adult human whole saliva evidenced three protein masses (M average 11,487 +/- 2, 11,301 +/- 2 and 22,362 +/- 3 Da) eluting in the 32.5-35.0 mwin range. Treatment in reducing conditions allowed establishing that they are S-derivatives of N-terminal acetylated cystatin B, namely its S-glutathionyl, S-cysteinyl and S-S dimer. The identification was confirmed by high resolution HPLC-ESI-MS-MS experiments on the intact naturally occurring proteins and their tryptic digests. S-unmodified cystatin B is rarely detectable in whole saliva of healthy adults (5 subjects out of 65) and its percentage does not overcome approximately 20% of total cystatin B (11 +/- 9%). In the majority of subjects (60 out of 65) the mean percentages of the S-modified derivatives were S-glutathionyl 53 +/- 13%, S-cysteinyl 15 +/- 5%, S-S 2-mer 32 +/- 13%. Variations of the percentages of these S-modified derivatives of cystatin B could be indicative of oral oxidative stress. As we are aware, this is the first time that S-glutathionylation and S-cysteinylation were described as extensive PTM of a salivary protein and the first time that these PTMs were detected in naturally occurring cystatin B. (C) 2011 Elsevier B.V. All rights reserved. (literal)
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