HPLC-ESI-MS and MS/MS structural characterization of multifucosylated N-glycoforms of the basic proline-rich protein IB-8a CON1(+) in human saliva (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• HPLC-ESI-MS and MS/MS structural characterization of multifucosylated N-glycoforms of the basic proline-rich protein IB-8a CON1(+) in human saliva (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/jssc.201101066 (literal)

Alternative label

• Cabras, Tiziana; Boi, Roberto; Pisano, Elisabetta; Iavarone, Federica; Fanali, Chiara; Nemolato, Sonia; Faa, Gavino; Castagnola, Massimo; Messana, Irene (2012)
  HPLC-ESI-MS and MS/MS structural characterization of multifucosylated N-glycoforms of the basic proline-rich protein IB-8a CON1(+) in human saliva
  in Journal of separation science (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Cabras, Tiziana; Boi, Roberto; Pisano, Elisabetta; Iavarone, Federica; Fanali, Chiara; Nemolato, Sonia; Faa, Gavino; Castagnola, Massimo; Messana, Irene (literal)

Pagina inizio

• 1079 (literal)

Pagina fine

• 1086 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 35 (literal)

Rivista

• Journal of separation science (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 9 (literal)

Note

• ISI Web of Science (WoS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Cagliari; Catholic University of the Sacred Heart; Catholic University of the Sacred Heart; University of Cagliari; University of Cagliari (literal)

Titolo

• HPLC-ESI-MS and MS/MS structural characterization of multifucosylated N-glycoforms of the basic proline-rich protein IB-8a CON1(+) in human saliva (literal)

Abstract

• This study describes the characterization of the glycan moieties and the peptide backbone of six glycoforms of IB-8a CON1+, a basic proline-rich protein present in human saliva. MS analyses on the intact glycoproteins before and after N-deglycosylation with PNGase F and high-resolution MS/MS sequencing by LTQ Orbitrap XL of peptides and glycopeptides from tryptic digests allowed the structural characterization of the glycan moieties and the polypeptide backbone, as well as to establish the glycosylation site at the asparagine residue at 98th position. Five of the glycoforms carry a biantennary N-linked glycan fucosylated in the innermost N-acetylglucosamine of the core and showing from zero to four additional fucoses in the antennal region. The sixth glycoform carries a monoantennary monofucosylated oligosaccharide. The glycoform cluster was detected on 28 of 71 adult saliva specimens. Level of fucosylation showed interindividual variability with the major relative abundance for the trifucosylated glycoform. Nonglycosylated IB-8a CON1+ and the variant IB-8a CON1-, lacking of the glycosylation site, have been also detected in human saliva. (literal)

Prodotto di

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Proteomica salivare (PM.P06.008.002) (Modulo)

Autore CNR

• MASSIMO CASTAGNOLA (Persona)

Insieme di parole chiave

• Keywords of "HPLC-ESI-MS and MS/MS structural characterization of multifucosylated N-glycoforms of the basic proline-rich protein IB-8a CON1(+) in human saliva" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Proteomica salivare (PM.P06.008.002) (Modulo)

Autore CNR di

• MASSIMO CASTAGNOLA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of separation science (Rivista)

Insieme di parole chiave di

• Keywords of "HPLC-ESI-MS and MS/MS structural characterization of multifucosylated N-glycoforms of the basic proline-rich protein IB-8a CON1(+) in human saliva" (Insieme di parole chiave)