The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well watered conditions but not under water stress. (Articolo in rivista)

Type
Label
  • The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well watered conditions but not under water stress. (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1104/pp.112.203455 (literal)
Alternative label
  • Perrone I., Gambino G., Chitarra W., Vitali M., Pagliarani C., Riccomagno N., Balestrini R., Kaldenhoff R., Uehlein N., Gribaudo I., Schubert A., Lovisolo C. (2012)
    The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well watered conditions but not under water stress.
    in Plant physiology (Bethesda)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Perrone I., Gambino G., Chitarra W., Vitali M., Pagliarani C., Riccomagno N., Balestrini R., Kaldenhoff R., Uehlein N., Gribaudo I., Schubert A., Lovisolo C. (literal)
Pagina inizio
  • 965 (literal)
Pagina fine
  • 977 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.plantphysiol.org/content/160/2/965 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 160 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1) I. Perrone, W. Chitarra, M. Vitali, C. Pagliarani, N. Riccomagno, A. Schubert, C. Lovisolo 1) Department of Agricultural, Forestry, and Food Sciences, University of Turin, 10095 Grugliasco, Italy: 2) G. Gambino, I. Gribaudo,, C. Lovisolo 2) Plant Virology Institute, National Research Council, Grugliasco Unit, 10095 Grugliasco, Italy; 3) R. Kaldenhoff, N. Uehlein 3) Darmstadt University of Technology, Applied Plant Science, D-64287 Darmstadt, Germany (literal)
Titolo
  • The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well watered conditions but not under water stress. (literal)
Abstract
  • We functionally characterized the grape (Vitis vinifera) VvPIP2;4N (for Plasma membrane Intrinsic Protein) aquaporin gene. Expression of VvPIP2;4N in Xenopus laevis oocytes increased their swelling rate 54-fold. Northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that VvPIP2;4N is the most expressed PIP2 gene in root. In situ hybridization confirmed root localization in the cortical parenchyma and close to the endodermis. We then constitutively overexpressed VvPIP2;4N in grape 'Brachetto', and in the resulting transgenic plants we analyzed (1) the expression of endogenous and transgenic VvPIP2;4N and of four other aquaporins, (2) whole-plant, root, and leaf ecophysiological parameters, and (3) leaf abscisic acid content. Expression of transgenic VvPIP2;4N inhibited neither the expression of the endogenous gene nor that of other PIP aquaporins in both root and leaf. Under well-watered conditions, transgenic plants showed higher stomatal conductance, gas exchange, and shoot growth. The expression level of VvPIP2;4N (endogenous + transgene) was inversely correlated to root hydraulic resistance. The leaf component of total plant hydraulic resistance was low and unaffected by overexpression of VvPIP2;4N. Upon water stress, the overexpression of VvPIP2;4N induced a surge in leaf abscisic acid content and a decrease in stomatal conductance and leaf gas exchange. Our results show that aquaporin-mediated modifications of root hydraulics play a substantial role in the regulation of water flow in well-watered grapevine plants, while they have a minor role upon drought, probably because other signals, such as abscisic acid, take over the control of water flow. (literal)
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