Insertion of a myc-tag within alpha-dystroglycan domains improves its biochemical and microscopic detection (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Insertion of a myc-tag within alpha-dystroglycan domains improves its biochemical and microscopic detection (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1186/1471-2091-13-14 (literal)

Alternative label

• Morlacchi, Simona; Sciandra, Francesca; Bigotti, Maria Giulia; Bozzi, Manuela; Huebner, Wolfgang; Galtieri, Antonio; Giardina, Bruno; Brancaccio, Andrea (2012)
  Insertion of a myc-tag within alpha-dystroglycan domains improves its biochemical and microscopic detection
  in BMC biochemistry (Online)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Morlacchi, Simona; Sciandra, Francesca; Bigotti, Maria Giulia; Bozzi, Manuela; Huebner, Wolfgang; Galtieri, Antonio; Giardina, Bruno; Brancaccio, Andrea (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 13 (literal)

Rivista

• BMC biochemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Note

• ISI Web of Science (WoS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Catholic University of the Sacred Heart; University of Messina; European Molecular Biology Laboratory (EMBL) (literal)

Titolo

• Insertion of a myc-tag within alpha-dystroglycan domains improves its biochemical and microscopic detection (literal)

Abstract

• Background: Epitope tags and fluorescent fusion proteins have become indispensable molecular tools for studies in the fields of biochemistry and cell biology. The knowledge collected on the subdomain organization of the two subunits of the adhesion complex dystroglycan (DG) enabled us to insert the 10 amino acids myc-tag at different locations along the alpha-subunit, in order to better visualize and investigate the DG complex in eukaryotic cells. (literal)
• Results: We have generated two forms of DG polypeptides via the insertion of the myc-tag 1) within a flexible loop (between a. a. 170 and 171) that separates two autonomous subdomains, and 2) within the C-terminal domain in position 500. Their analysis showed that double-tagging (the beta-subunit is linked to GFP) does not significantly interfere with the correct processing of the DG precursor (pre-DG) and confirmed that the alpha-DG N-terminal domain is processed in the cell before alpha-DG reaches its plasma membrane localization. In addition, myc insertion in position 500, right before the second Ig-like domain of alpha-DG, proved to be an efficient tool for the detection and pulling-down of glycosylated alpha-DG molecules targeted at the membrane. (literal)

Prodotto di

• Modellistica molecolare di proteine legate a processi patologici (PM.P01.026.002) (Modulo)
• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)

Autore CNR

• ANDREA BRANCACCIO (Unità di personale interno)
• simona morlacchi (Persona)
• BRUNO GIARDINA (Persona)
• FRANCESCA SCIANDRA (Unità di personale interno)

Incoming links:

Autore CNR di

• ANDREA BRANCACCIO (Unità di personale interno)
• BRUNO GIARDINA (Persona)
• simona morlacchi (Persona)
• FRANCESCA SCIANDRA (Unità di personale interno)

Prodotto

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Modellistica molecolare di proteine legate a processi patologici (PM.P01.026.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• BMC biochemistry (Rivista)