Altered expression level of Escherichia coli proteins in response to treatment with the antifouling agent zosteric acid sodium salt (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Altered expression level of Escherichia coli proteins in response to treatment with the antifouling agent zosteric acid sodium salt (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1111/j.1462-2920.2011.02678.x (literal)

Alternative label

• Villa, Federica; Remelli, William; Forlani, Fabio; Vitali, Alberto; Cappitelli, Francesca (2012)
  Altered expression level of Escherichia coli proteins in response to treatment with the antifouling agent zosteric acid sodium salt
  in Environmental microbiology (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Villa, Federica; Remelli, William; Forlani, Fabio; Vitali, Alberto; Cappitelli, Francesca (literal)

Pagina inizio

• 1753 (literal)

Pagina fine

• 1761 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 14 (literal)

Rivista

• Environmental microbiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 7 (literal)

Note

• ISI Web of Science (WoS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Milan; University of Milan; Catholic University of the Sacred Heart (literal)

Titolo

• Altered expression level of Escherichia coli proteins in response to treatment with the antifouling agent zosteric acid sodium salt (literal)

Abstract

• Zosteric acid sodium salt is a powerful antifouling agent. However, the mode of its antifouling action has not yet been fully elucidated. Whole cell proteome of Escherichia coli was analysed to study the different protein patterns expressed by the surface-exposed planktonic cells without and with sublethal concentrations of the zosteric acid sodium salt. Proteomic analysis revealed that at least 27 proteins showed a significant (19 upregulated and 8 downregulated, P < 0.001) altered expression level in response to the antifoulant. The proteomic signatures of zosteric acid sodium salt-treated cells are characterized by stress-associated (e.g. AhpC, OsmC, SodB, GroES, IscU, DnaK), motility-related (FliC), quorum-sensing-associated (LuxS) and metabolism/biosynthesis-related (e.g. PptA, AroA, FabD, FabB, GapA) proteins. Consistent with the overexpression of LuxS enzyme, the antifouling agent increased autoinducer-2 (AI-2) concentration by twofold. Moreover, treated cells experienced a statistically significant but modest increase of reactive oxygen species (+ 23%), tryptophanase (1.2-fold) and indole (1.2-fold) synthesis. Overall, our data suggest that zosteric acid sodium salt acts as environmental cue leading to global stress on E. coli cells, which favours the expression of various protective proteins, the AI-2 production and the synthesis of flagella, to escape from adverse conditions. (literal)

Prodotto di

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Proteomica salivare (PM.P06.008.002) (Modulo)

Autore CNR

• ALBERTO VITALI (Unità di personale interno)

Incoming links:

Autore CNR di

• ALBERTO VITALI (Unità di personale interno)

Prodotto

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Proteomica salivare (PM.P06.008.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Environmental microbiology (Rivista)