Pulsatile protein release and protection using radiation-crosslinked polypeptide hydrogel delivery devices (Articolo in rivista)

Type
Label
  • Pulsatile protein release and protection using radiation-crosslinked polypeptide hydrogel delivery devices (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.reactfunctpolym.2010.11.030 (literal)
Alternative label
  • LoPresti C; Vetri V; Ricca M; Fodera V; Tripodo G; Spadaro G; Dispenza C (2011)
    Pulsatile protein release and protection using radiation-crosslinked polypeptide hydrogel delivery devices
    in Reactive & functional polymers (Print); ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, AMSTERDAM (Paesi Bassi)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • LoPresti C; Vetri V; Ricca M; Fodera V; Tripodo G; Spadaro G; Dispenza C (literal)
Pagina inizio
  • 155 (literal)
Pagina fine
  • 167 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.sciencedirect.com/science/article/pii/S1381514810002257# (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 71 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Dipartimento di Ingegneria Chimica dei Processi e dei Materiali, Università di Palermo, Viale delle Scienze Ed. 6, 90100 Palermo, Italy; Dipartimento di Scienze Fisiche ed Astronomiche, Università di Palermo, Via Archirafi 36, 90123 Palermo, Italy; CNR - Istituto di Biofisica, U.O. Via U. La Malfa 153, 90146 Palermo, Italy; Dipartimento di Chimica e Tecnologie Farmaceutiche, Università di Palermo, Via Archirafi 32, 90123 Palermo, Italy (literal)
Titolo
  • Pulsatile protein release and protection using radiation-crosslinked polypeptide hydrogel delivery devices (literal)
Abstract
  • In the recent years recombinant technology has identified numerous protein based therapeutics. Their effective delivery, though, can be challenging due to the poor stability of most proteins along their pathway to the target site in the body. Hydrogels have been identified as good candidates for protein encapsulation and delivery thanks to both material and manufacturing process advantages. In this work we propose high energy irradiation as a synthetic methodology of choice to engineer hydrogel-based delivery devices for encapsulation and pulsatile release of proteins, triggered by pH, and for prevention of their denaturation when encapsulated. In particular, maleic anhydride functionalised poly(N-2-HydroxyEthyl)-DL-Aspartamide (PHEA-MA) hydrogels have been crosslinked without the use of toxic reagents or catalysts and in mild conditions via gamma irradiation. At the variance of the irradiation conditions, hydrogels with dramatically different crosslinked structure, thus rheological properties and swelling behaviour, have been obtained. The ability to swell and shrink cyclically upon repeated pH jumps and the absence of cytotoxicity have been demonstrated for all the hydrogels produced. Moreover some of the variants exhibited full degradability at 37 °C with degradation products that are not-toxic for the cell. Depending on the networks average mesh size, as derived by the treatment of rheological data with simple rubber elasticity equations, with respect to the characteristic dimension of the chosen model protein, substantial loading of the protein and its retention or release, controlled by pH, have been achieved. These results, coupled with the versatility of the synthetic platform, suggest the possibility to use these materials as components of intelligent/programmable devices specifically designed as to release theoretically any protein based therapeutic. (literal)
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