Proteomic characterization of a mouse model of familial Danish dementia. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Proteomic characterization of a mouse model of familial Danish dementia. (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1155/2012/728178 (literal)

Alternative label

• Vitale M, Renzone G, Matsuda S, Scaloni A, D'Adamio L, Zambrano N. (2012)
  Proteomic characterization of a mouse model of familial Danish dementia.
  in Journal of Biomedicine and Biotechnology (Print); Hindawi Publishing Corporation, Cairo (Egitto)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Vitale M, Renzone G, Matsuda S, Scaloni A, D'Adamio L, Zambrano N. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3350990/ (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 2012:728178 (literal)

Rivista

• Journal of Biomedicine and Biotechnology (Print) (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dipartimento di Biochimica e Biotecnologie Mediche, Universita degli Studi di Napoli Federico II, 80131 Napoli, Italy CEINGE Biotecnologie Avanzate, 80145 Napoli, Italy Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Napoli, Italy Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10462, USA (literal)

Titolo

• Proteomic characterization of a mouse model of familial Danish dementia. (literal)

Abstract

• A dominant mutation in the ITM2B/BRI2 gene causes familial Danish dementia (FDD) in humans. To model FDD in animal systems, a knock-in approach was recently implemented in mice expressing a wild-type and mutant allele, which bears the FDD-associated mutation. Since these FDD(KI) mice show behavioural alterations and impaired synaptic function, we characterized their synaptosomal proteome via two-dimensional differential in-gel electrophoresis. After identification by nanoliquid chromatography coupled to electrospray-linear ion trap tandem mass spectrometry, the differentially expressed proteins were classified according to their gene ontology descriptions and their predicted functional interactions. The Dlg4/Psd95 scaffold protein and additional signalling proteins, including protein phosphatases, were revealed by STRING analysis as potential players in the altered synaptic function of FDD(KI) mice. Immunoblotting analysis finally demonstrated the actual downregulation of the synaptosomal scaffold protein Dlg4/Psd95 and of the dual-specificity phosphatase Dusp3 in the synaptosomes of FDD(KI) mice. (literal)

Editore

• Hindawi Publishing Corporation (Editore)

Prodotto di

• Metodologie genomiche e proteomiche applicate al sistema zootecnico ed agroindustriale (AG.P01.015.001) (Modulo)
• Institute for animal production system in mediterranean environment (ISPAAM) (Istituto)

Autore CNR

• GIOVANNI RENZONE (Unità di personale esterno)
• ANDREA SCALONI (Persona)

Incoming links:

Autore CNR di

• ANDREA SCALONI (Persona)
• GIOVANNI RENZONE (Unità di personale esterno)

Prodotto

• Institute for animal production system in mediterranean environment (ISPAAM) (Istituto)
• Metodologie genomiche e proteomiche applicate al sistema zootecnico ed agroindustriale (AG.P01.015.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of Biomedicine and Biotechnology (Print) (Rivista)

Editore di

• Hindawi Publishing Corporation (Editore)