Photorespiratory Metabolism and Nodule Function: Behavior of Lotus japonicus Mutants Deficient in Plastid Glutamine Synthetase (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Photorespiratory Metabolism and Nodule Function: Behavior of Lotus japonicus Mutants Deficient in Plastid Glutamine Synthetase (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1094/MPMI-07-11-0200 (literal)

Alternative label

• García-Calderón M; Chiurazzi M; Espuny MR; Márquez AJ. (2012)
  Photorespiratory Metabolism and Nodule Function: Behavior of Lotus japonicus Mutants Deficient in Plastid Glutamine Synthetase
  in Molecular plant-microbe interactions
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• García-Calderón M; Chiurazzi M; Espuny MR; Márquez AJ. (literal)

Pagina inizio

• 211 (literal)

Pagina fine

• 219 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 25 (literal)

Rivista

• Molecular plant-microbe interactions (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 2 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Univ Seville, Dept Bioquim Vegetal & Biol Mol, E-41012 Seville, Spain Inst Genet & Biophys A Buzzati Traverso, I-80131 Naples, Italy Univ Seville, Dept Microbiol, Fac Biol, E-41012 Seville, Spain (literal)

Titolo

• Photorespiratory Metabolism and Nodule Function: Behavior of Lotus japonicus Mutants Deficient in Plastid Glutamine Synthetase (literal)

Abstract

• Two photorespiratory mutants of Lotus japonicus deficient in plastid glutamine synthetase (GS(2)) were examined for their capacity to establish symbiotic association with Mesorhizobium Ion bacteria. Biosynthetic glutamine synthetase (GS) activity was reduced by around 40% in crude nodule extracts from mutant plants as compared with the wild type (WT). Western blot analysis further confirmed the lack of GS(2) polypeptide in mutant nodules. The decrease in GS activity affected the nodular carbon metabolism under high CO(2) (suppressed photorespiration) conditions, although mutant plants were able to form nodules and fix atmospheric nitrogen. However, when WT and mutant plants were transferred to an ordinary air atmosphere (photorespiratory active conditions) the nodulation process and nitrogen fixation were substantially affected, particularly in mutant plants. The number and fresh weight of mutant nodules as well as acetylene reduction activity showed a strong inhibition compared with WT plants. Optical microscopy studies from mutant plant nodules revealed the anticipated senescence phenotype linked to an important reduction in starch and sucrose levels. These results show that, in Lotus japonicus, photorespiration and, particularly, GS(2) deficiency result in profound limitations in carbon metabolism that affect the nodulation process and nitrogen fixation. (literal)

Prodotto di

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Le interazioni benefiche fra organismi: dalla lotta biologica alla messa a punto di strumenti per un'agricoltura sostenibile. (AG.P01.005.001) (Modulo)

Autore CNR

• MAURIZIO CHIURAZZI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Photorespiratory Metabolism and Nodule Function: Behavior of Lotus japonicus Mutants Deficient in Plastid Glutamine Synthetase" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Le interazioni benefiche fra organismi: dalla lotta biologica alla messa a punto di strumenti per un'agricoltura sostenibile. (AG.P01.005.001) (Modulo)

Autore CNR di

• MAURIZIO CHIURAZZI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Molecular plant-microbe interactions (Rivista)

Insieme di parole chiave di

• Parole chiave di "Photorespiratory Metabolism and Nodule Function: Behavior of Lotus japonicus Mutants Deficient in Plastid Glutamine Synthetase" (Insieme di parole chiave)