Time course and distribution of TUNEL nuclear positivity of cardiomyocytes in a chronic rat model of coronary occlusion with and without reperfusion (Abstract/Poster in rivista)

Type
Label
  • Time course and distribution of TUNEL nuclear positivity of cardiomyocytes in a chronic rat model of coronary occlusion with and without reperfusion (Abstract/Poster in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1093/cvr/cvq174 (literal)
Alternative label
  • G. Pelosi ; M.Matteucci ; C. Kusmic ; N. Vesentini ; F. Piccolomini ; F. Viglione ; MG. Trivella ; A. L'Abbate (2010)
    Time course and distribution of TUNEL nuclear positivity of cardiomyocytes in a chronic rat model of coronary occlusion with and without reperfusion
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • G. Pelosi ; M.Matteucci ; C. Kusmic ; N. Vesentini ; F. Piccolomini ; F. Viglione ; MG. Trivella ; A. L'Abbate (literal)
Pagina inizio
  • S 76 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 87 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 1 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1 (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Fisiologia Clinica C.N.R. Pisa SSSA Pisa (literal)
Titolo
  • Time course and distribution of TUNEL nuclear positivity of cardiomyocytes in a chronic rat model of coronary occlusion with and without reperfusion (literal)
Abstract
  • Occlusion of a coronary artery either permanent or followed by reperfusion is known to cause irreversible myocellular death mainly by \"oncotic\" modality. DNA fragmentation by in situ terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) has been frequently detected at light microscopy (LM) in oncotic cells and believed to be an index of a caspase-mediated \"apoptotic\" type of cellular death, although typical apoptotic ultrastructural features have never been observed. The contribution of this DNA fragmentation- associated oncotic myocellular death to overall irreversible myocardial necrosis after ischemia and ischemia reperfusion is still questionable and its time course unknown. Purpose: To assess the time course and distribution of TUNEL nuclear positivity of cardiomyocytes within the infarcted and ischemic-reperfused myocardium of rat heart over a 3 day time span. Methods: Male rats underwent permanent (I, n ¼ 12) or transient (I-R, 30min occlusion followed by reperfusion, n ¼ 12) ligation of the left anterior descending coronary artery and hearts explanted at 1h (groups 1h I, 1h I-R), 1 day (groups 1d I, 1d I-R) and 3 days (groups 3d I and 3d I-R). Sham-operated animals served as controls (C, n ¼ 6). Histology (H&E and Masson trichromic stain) and TUNEL assay were performed on 10 consecutive serial slices at two levels distal to the ligature for LM morphometric assessment of necrosis and nuclear TUNEL analysis. Results: In groups 1h I and 1h I-R neither cellular necrosis or TUNEL positivity could be detected both in risk and control areas as well as in C hearts. In group 1d I contraction bands in the periphery and coagulation necrotic myocells in the central part of infarction showed 30 + 5% and 45 + 3% of TUNEL positive nuclei respectively. In group 1d I-R 50 + 5% of contraction band necrotic cells had TUNEL positive nuclei. In groups 3d I and 3d I-R most of the still visible nuclei of necrotic myocytes were positive (94 + 5 and 95 + 4% respectively). TUNEL positivity was never observed in myocytes without LM evidence of cellular necrosis. Conclusion: The present findings demonstrate that DNA fragmentation: a) invariably coexists with myocellular necrosis prior to complete nuclear demise over a 3 day time span, b) it has a comparable incidence in I and I-R groups, c) it persists at 3 days with an even higher incidence. The possibility that TUNEL positivity could identify a specific caspase-dependent subpopulation of irreversibly damaged cardiomyocytes within the context of ischemic and ischemic-reperfusion necrosis is suggested. (literal)
Prodotto di
Autore CNR

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it