Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.ab.2005.08.004 (literal)

Alternative label

• C. Peanoa, R. Bordoni, M. Gulli, A. Mezzelani, M.C. Samson, G. De Bellis, N. Marmiroli (2005)
  Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods
  in Analytical biochemistry (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• C. Peanoa, R. Bordoni, M. Gulli, A. Mezzelani, M.C. Samson, G. De Bellis, N. Marmiroli (literal)

Pagina inizio

• 90 (literal)

Pagina fine

• 100 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 346 (literal)

Rivista

• Analytical biochemistry (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute of Biomedical Technologies, National Research Council, Via Fratelli Cervi 93, Segrate, Milano 20090, Italy;Department of Environmental Sciences, University of Parma, Section of Genetics and Environmental Biotechnologies, Viale delle Scienze 11/A, Parma 43100, Italy (literal)

Titolo

• Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods (literal)

Abstract

• : A multiplex polymerase chain reaction (PCR) system was developed for the simultaneous detection of target sequences in genetically modified soybean (Roundup Ready) and maize (MON810, Bt76, Bt11, and GA21). Primer pairs were designed to amplify the junction regions of the transgenic constructs analyzed and the endogenous genes of soybean (lectin) and maize (zein) were included as internal control targets to assess the efficiency of all reactions. This multiplex PCR has constituted the basis for an efficient platform for genetically modified organism traceability based on microarray technology. In particular, the ligation detection reaction combined to a universal array approach, using the multiplex PCR as target, was applied. High specificity and sensitivity were obtained. (literal)

Autore CNR

• GIANLUCA DE BELLIS (Persona)
• ROBERTA BORDONI (Unità di personale interno)
• CLELIA PEANO (Unità di personale interno)
• ALESSANDRA MARIA MEZZELANI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ROBERTA BORDONI (Unità di personale interno)
• GIANLUCA DE BELLIS (Persona)
• ALESSANDRA MARIA MEZZELANI (Unità di personale interno)
• CLELIA PEANO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Analytical biochemistry (Rivista)

Insieme di parole chiave di

• Parole chiave di "Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods" (Insieme di parole chiave)