http://www.cnr.it/ontology/cnr/individuo/prodotto/ID18623

Putative glycine receptors in Hydra: a biochemical and behavioural study (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Putative glycine receptors in Hydra: a biochemical and behavioural study (Articolo in rivista) (literal)

Anno

2001-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1046/j.0953-816x.2001.01792.x (literal)

Alternative label

Pierobon P.1, Minei R.2, Porcu P.3, Sogliano C.4, Tino A.5, Marino G.6, Biggio G.7, Concas A.8 (2001)
Putative glycine receptors in Hydra: a biochemical and behavioural study
in European journal of neuroscience (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Pierobon P.1, Minei R.2, Porcu P.3, Sogliano C.4, Tino A.5, Marino G.6, Biggio G.7, Concas A.8 (literal)

Pagina inizio

1659 (literal)

Pagina fine

1666 (literal)

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Il lavoro ha ottenuto la copertina della rivista (literal)

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http://onlinelibrary.wiley.com/doi/10.1046/j.0953-816x.2001.01792.x/abstract (literal)

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14 (literal)

Rivista

European journal of neuroscience (Rivista)

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IF 2002: 4,16. Settore disciplinare Neuroscienze (categorie ISI-CRUI). La rivista è nella fascia di eccellenza del settore (da 3,74 a 24,09). IF ricalcolato in base al rango: 0,838. ImmediacyIndex 0,36, Cit Half-Life 4,1. (literal)

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10 (literal)

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Individuazione e caratterizzazione biochimica e funzionale di recettori alla glicina in Hydra (Cnidari). Il lavoro fornisce la prima evidenza dell'esistenza di tali recettori anche in Invertebrati, smentendo l'opinione corrente secondo la quale essi sarebbero tipici dei sistemi nervosi più evoluti. (literal)

Note

PubMe (literal)
ISI Web of Science (WOS) (literal)

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1,2,5,6: ICIB-CNR, Pozzuoli (NA) Italy 3,4,7,8: Dip. Biologia sperimentale, Università degli Studi di Cagliari, Italy (literal)

Titolo

Putative glycine receptors in Hydra: a biochemical and behavioural study (literal)

Abstract

Glycine acts as an inhibitory transmitter in the lower brain stem and spinal cord of vertebrate species, while very few data are yet available to support a similar role in invertebrate nervous systems. Here we report the identification and characterisation of glycine receptors in the freshwater polyp Hydra vulgaris (Cnidaria, Hydrozoa) by biochemical and behavioural studies. Saturation experiments revealed the occurrence of one population of binding sites for [3H]strychnine of nanomolar affinity (KD = 33 nM) and low capacity (Bmax = 79 fmol/mg protein). The addition of glycine or taurine (0.1 mM to 1 mM) produced a dose-dependent inhibition of [3H]strychnine binding. b-alanine (0.1 mM to 1 mM) did not significantly affect [3H]strychnine binding. The pharmacological properties of these receptors compare with those of vertebrate glycine receptors. Stimulation of hydra polyps by reduced glutathione resulted in a significant increase of the duration of mouth opening in the presence of glycine, taurine or b-alanine. The enhancement of the response was related both to amino acid (10-100 mM) and to glutathione concentration (1-10 mM). The effects of glycine or its agonists were suppressed by strychnine (1-10 mM). D-serine, the glycine agonist at the vertebrate NMDA receptor, produced opposite effects to those of glycine. The effects of D-serine were suppressed by 5,7-dichlorokynurenic acid but not by strychnine. In vitro, [3H]strychnine binding was not displaced by D-serine. These results indicate a dual action of glycine in hydra tissues. The hypothesis that NMDA receptors may also be present in this elementary nervous system is proposed. (literal)

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